UTEX 2164.
Nannochloropsis oculata


Condition: Axenic

Medium Type: Agar Slant


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View General Maintenance Conditions


Algae Details

UTEX Number: 2164.
Class: Eustigmatophyceae
Strain: Nannochloropsis oculata (Droop) Hibberd
Medium: Modified Artificial Seawater Medium (MASM)
Origin: Isle of Cumbrae, Scotland
Description of Location: brackish rockpool
GPS:
Type Culture: No
Collection: Summer of 1953
Isolation: M.R. Droop
Isolator Number: Millport 66 aka SMBA 66
Deposition: A.R. Loeblich III (04/1977)
Relatives: CCAP 849/1; SAG 38.85; CCMP525 aka CCMP 66, WHOI 66
Also Known As: Nannochloris oculata
Notes:

Axenic isolate from LB 2164; 1981 renamed (Hibberd 1981); produces highly unsaturated fatty acids [C20:5 & C22:6] (Thompson et al. 1988); (Antia et al. 1975)

The agar-maintained version of 2164 does not transition easily to liquid media.The liquid media version (LB 2164) is recommended for immediate growth in liquid media.

General Long-Term Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12:12 Light/Dark Cycle

To reduce the risk of losing newly acquired cultures, it is highly recommended to initially duplicate the Collection's long-term maintenance conditions. Please note that these general maintenance conditions are not designed for optimal algal growth rates or large quantities. For information on the best growth conditions and media, consult other literature or conduct your own experiments. Once a stock culture is established, subcultures can be used to test other conditions.

You can find additional details on the long-term culture maintenance conditions on the Culture Maintenance Guides page.


Preparation of Living Algal Strains For Orders

Agar cultures are transferred to fresh agar slants in 20-mm glass screwcap tubes. After quality control (QC) checks via microscopy are completed, the tubes are cultured under a diurnal light cycle for a minimum of three (3) days.

Cultures are sealed and packaged in a temperature-regulating mailer and shipped the same day.

UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture and only guarantees the identity of the organism as specified in the strain history.