UTEX 753
Euglena gracilis

UTEX Prefix Labeling System

LB Xenic strain maintained in liquid media
B Xenic strain maintained on an agar slant
'no prefix' Axenic strain, free of any contaminants and maintained on an agar slant


Algae Detail

UTEX Number: 753
Class: Euglenophyceae
Strain: Euglena gracilis
Media: Euglena Medium
Origin:
Description of Location:
Type Culture: No
Collection: DeSaedeleer
Isolation: E.G. Pringsheim (1950)
Isolator Number:
Deposition: S.H. Hutner (1952-5)
Relatives: CCAP 1224/5Z; SAG 1224-5/25; ATCC 12894 aka ATCC 12716 [dec.]; NIVA 1/79; UTCC 95; IAM E-6; NIES 48
Also Known As:
Notes: to Hutner from E.A. George as the Cambridge "Z"-strain; aka Pringsheim's 25 (Pringsheim & Pringsheim 1952); bioassay of vitamin B12 (Hutner et al. 1956); paramylon synthesis (Kiss et al. 1988); photosystem/ATPase-complex/CF1 assembly (Brandt & Ostermann 1988); heavy metal binding complexes (Gekeler et al. 1988); fatty acids, fatty alcohols & sugars/assimilation of (Hosotani et al. 1988); cobalamin-binding proteins/uptake system (Watanabe [F.] et al. 1988a); extracellular cobalamin binding-proteins (Watanabe [F.] et al. 1988b); chloroplast genome (Cushman et al. 1988); chloroplast genome/ribosomal protein genes (Christopher et al. 1988); ultrastructure/ tetracaine/ intracellular Ca++ (Vannini et al. 1988); uroporphyrinogen dicarboxylase (Juknat et al. 1989); UTP compartmentation/cytoplasmic RNAs (Karnahl & Wasternack 1989); cyanobacterin disrupts thylakoid (Gleason 1990)

General Maintenance Conditions

Temperature: 20 °C
Light source: cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.

** Estimate for transfer interval based on culturing conditions utilized at UTEX (long-term maintenance).

Preparation of Living Algal Strains

Aliquots of algal strains that are maintained on agar slants are transferred to the surface of fresh slants in 20 mm diameter glass screw-captubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears. Approximately 15 mL aliquots of strains that are maintained as liquid cultures are transferred to screw-cap tubes on the day of shipment. Agar and liquid cultures for a customer are packaged within a Styrofoam™ box and shipped on the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

  • Algae Detail

    UTEX Number: 753
    Class: Euglenophyceae
    Strain: Euglena gracilis
    Media: Euglena Medium
    Origin:
    Description of Location:
    Type Culture: No
    Collection: DeSaedeleer
    Isolation: E.G. Pringsheim (1950)
    Isolator Number:
    Deposition: S.H. Hutner (1952-5)
    Relatives: CCAP 1224/5Z; SAG 1224-5/25; ATCC 12894 aka ATCC 12716 [dec.]; NIVA 1/79; UTCC 95; IAM E-6; NIES 48
    Also Known As:
    Notes: to Hutner from E.A. George as the Cambridge "Z"-strain; aka Pringsheim's 25 (Pringsheim & Pringsheim 1952); bioassay of vitamin B12 (Hutner et al. 1956); paramylon synthesis (Kiss et al. 1988); photosystem/ATPase-complex/CF1 assembly (Brandt & Ostermann 1988); heavy metal binding complexes (Gekeler et al. 1988); fatty acids, fatty alcohols & sugars/assimilation of (Hosotani et al. 1988); cobalamin-binding proteins/uptake system (Watanabe [F.] et al. 1988a); extracellular cobalamin binding-proteins (Watanabe [F.] et al. 1988b); chloroplast genome (Cushman et al. 1988); chloroplast genome/ribosomal protein genes (Christopher et al. 1988); ultrastructure/ tetracaine/ intracellular Ca++ (Vannini et al. 1988); uroporphyrinogen dicarboxylase (Juknat et al. 1989); UTP compartmentation/cytoplasmic RNAs (Karnahl & Wasternack 1989); cyanobacterin disrupts thylakoid (Gleason 1990)

  • General Maintenance Conditions

    Temperature: 20 °C
    Light source: cool-white fluorescent lamps
    Intensity: 3200 lux (maximum)
    Periodicity: 12/12 h L/D

    Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.





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