UTEX 646
Phaeodactylum tricornutum

ATTENTION:

Due to the upcoming 2018 Winter Holiday closures, orders placed after the stated deadlines will not ship until 2019 (review expected shipping estimates).

UTEX Prefix Labeling System

LB Xenic strain maintained in liquid media
B Xenic strain maintained on an agar slant
'no prefix' Axenic strain, free of any contaminants and maintained on an agar slant


Algae Detail

UTEX Number:  646
Class:  Bacillariophyceae
Strain:  Phaeodactylum tricornutum
Media:  F/2 Medium
Origin:  Segelskär, Finland
Description of Location: 
Type Culture:  No
Collection: 
Isolation:  M.R. Droop (1951)
Isolator Number: 
Deposition:  CCAP (3/55)
Relatives:  ?UTEX 2089; CCAP 1052/6 aka SMBA 14 [?Millport 14]; SAG 1090-6; NIBB 3009 aka ORI-B15N, NIBB 3010
Also Known As:  Nitzschia closterium f. minutissima aka T-form
Notes:  1974 renamed by J.C. Lewin (pers. Comm.); light harvesting pigment proteins, monospecific antibodies (Friedman & Alberte 1987); guanine metabolism (Allison & Syrett 1987); urea metabolism/nickel & nitrogen deprivation effect (Syrett & Peplinska 1988); antifungal activity (Kellam et al. 1989); lipid content (Yongmanitchae & Ward 1991); (Lewin 1958); In 1974, J.C. Lewin (pers. Comm.) identified some of our Nitzchia cultures as Phaeodactylum [see UTEX 640, 642, 646 & 2089] with the following explanation: "Oval cells of Phaeodactylum occur in cultures kept on agar. There is a gradual transformation back to the fusiform state, after oval cells are inoculated into liquid medium. Sometimes, people who don't know about this unusual life style may end up being fooled."

General Maintenance Conditions

Temperature: 20 °C
Light source: cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.

** Estimate for transfer interval based on culturing conditions utilized at UTEX (long-term maintenance).

Preparation of Living Algal Strains

Aliquots of algal strains that are maintained on agar slants are transferred to the surface of fresh slants in 20 mm diameter glass screw-captubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears. Approximately 15 mL aliquots of strains that are maintained as liquid cultures are transferred to screw-cap tubes on the day of shipment. Agar and liquid cultures for a customer are packaged within a Styrofoam™ box and shipped on the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

  • Algae Detail

    UTEX Number:  646
    Class:  Bacillariophyceae
    Strain:  Phaeodactylum tricornutum
    Media:  F/2 Medium
    Origin:  Segelskär, Finland
    Description of Location: 
    Type Culture:  No
    Collection: 
    Isolation:  M.R. Droop (1951)
    Isolator Number: 
    Deposition:  CCAP (3/55)
    Relatives:  ?UTEX 2089; CCAP 1052/6 aka SMBA 14 [?Millport 14]; SAG 1090-6; NIBB 3009 aka ORI-B15N, NIBB 3010
    Also Known As:  Nitzschia closterium f. minutissima aka T-form
    Notes:  1974 renamed by J.C. Lewin (pers. Comm.); light harvesting pigment proteins, monospecific antibodies (Friedman & Alberte 1987); guanine metabolism (Allison & Syrett 1987); urea metabolism/nickel & nitrogen deprivation effect (Syrett & Peplinska 1988); antifungal activity (Kellam et al. 1989); lipid content (Yongmanitchae & Ward 1991); (Lewin 1958); In 1974, J.C. Lewin (pers. Comm.) identified some of our Nitzchia cultures as Phaeodactylum [see UTEX 640, 642, 646 & 2089] with the following explanation: "Oval cells of Phaeodactylum occur in cultures kept on agar. There is a gradual transformation back to the fusiform state, after oval cells are inoculated into liquid medium. Sometimes, people who don't know about this unusual life style may end up being fooled."
  • General Maintenance Conditions

    Temperature: 20 °C
    Light source: cool-white fluorescent lamps
    Intensity: 3200 lux (maximum)
    Periodicity: 12/12 h L/D

    Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.





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