UTEX 646
Phaeodactylum tricornutum


Condition: Axenic

Medium Type: Agar Slant


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View General Maintenance Conditions


Algae Details

UTEX Number: 646
Class: Bacillariophyceae
Strain: Phaeodactylum tricornutum
Media: F/2 Medium
Origin: Segelskí_r, Finland
Description of Location:
Type Culture: No
Collection:
Isolation: M.R. Droop (1951)
Isolator Number:
Deposition: CCAP (3/55)
Relatives: ?UTEX 2089; CCAP 1052/6 aka SMBA 14 [?Millport 14]; SAG 1090-6; NIBB 3009 aka ORI-B15N, NIBB 3010
Also Known As: Nitzschia closterium f. minutissima aka T-form
Notes: 1974 renamed by J.C. Lewin (pers. Comm.); light harvesting pigment proteins, monospecific antibodies (Friedman & Alberte 1987); guanine metabolism (Allison & Syrett 1987); urea metabolism/nickel & nitrogen deprivation effect (Syrett & Peplinska 1988); antifungal activity (Kellam et al. 1989); lipid content (Yongmanitchae & Ward 1991); (Lewin 1958); In 1974, J.C. Lewin (pers. Comm.) identified some of our Nitzchia cultures as Phaeodactylum [see UTEX 640, 642, 646 & 2089] with the following explanation: "Oval cells of Phaeodactylum occur in cultures kept on agar. There is a gradual transformation back to the fusiform state, after oval cells are inoculated into liquid medium. Sometimes, people who don't know about this unusual life style may end up being fooled."

General Long-Term Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12:12 Light/Dark Cycle

To reduce the risk of losing newly acquired cultures, it is highly recommended to initially duplicate the Collection's long-term maintenance conditions. Please note that these general maintenance conditions are not designed for optimal algal growth rates or large quantities. For information on the best growth conditions and media, consult other literature or conduct your own experiments. Once a stock culture is established, subcultures can be used to test other conditions.

You can find additional details on the long-term culture maintenance conditions on the Culture Maintenance Guides page.


Preparation of Living Algal Strains For Orders

Agar cultures are transferred to fresh agar slants in 20-mm glass screwcap tubes. After quality control (QC) checks via microscopy are completed, the tubes are cultured under a diurnal light cycle for a minimum of three (3) days.

Cultures are sealed and packaged in a temperature-regulating mailer and shipped the same day.

UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture and only guarantees the identity of the organism as specified in the strain history.


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