UTEX 640
Phaeodactylum tricornutum


Condition: Axenic

Medium Type: Agar Slant


View Algae Details

View Cryopreservation Conditions


Algae Details

UTEX Number: 646
Class: Bacillariophyceae
Strain: Phaeodactylum tricornutum
Media: Soil Extract + Sodium Metasilicate Medium (SE+Si)
Origin:
Description of Location:
Type Culture: No
Collection:
Isolation: M.B. Allen
Isolator Number:
Deposition: CCAP (3/55)
Relatives: UTEX 642, UTEX 2090; CCAP 1052/1B aka Barker's HMS Z912 from the Plymouth strain, SMBA 307, CCAP 1052/1A; SAG 1090-1b, SAG 1090-1a; SAG 1090-1a; Plymouth 100; IAM B-9, IAM B- 15; NIBB 3002
Also Known As: Nitzschia closterium
Notes: this is a marine isolate growing in freshwater medium; 1976 renamed (George 1976); high EPA/lipid content (Yongmanitchae & Ward 1991); (Lewin 1958, Lewin & Lewin 1960); In 1974, J.C. Lewin (pers. Comm.) identified some of our Nitzchia cultures as Phaeodactylum [see UTEX 640, 642, 646 & 2089] with the following explanation: "Oval cells of Phaeodactylum occur in cultures kept on agar. There is a gradual transformation back to the fusiform state, after oval cells are inoculated into liquid medium. Sometimes, people who don't know about this unusual life style may end up being fooled."

Strains that are maintained only in cryopreservation (designated 'cryo only') require a minimum of 4-weeks to grow out before shipment.


Cryopreservation Conditions

Temperature: -190 °C
Storage: Vapor phase of liquid nitrogen dewars

Cryopreservation stabilizes genomic integrity, preserves culture quality, minimizes maintenance costs and reduces the risk of catastrophic loss. A broad variety of techniques employed at the CCAP, at UTEX, and elsewhere, can be found in Day, J.G. and Brand, J.J. Cryopreservation Methods for Maintaining Microalgal Cultures, in Algal Culturing Techniques, ed. R. A. Andersen, Elsevier, Amsterdam, 2006. A relatively simple method that uses a minimum of specialized equipment, yet allows a large variety of microalgae to be cryopreserved with high viability, can be found on the Cryopreservation of Microalgae page.


Preparation of Strains Maintained Only In Cryopreservation For Orders

Strains that are maintained only in cryopreservation (cryo only) require a minimum of 4-weeks to thaw, grow out, and check viability before shipment. An estimated shipping date will be emailed when purchased. Updates via email will be sent if the strain is ready sooner or needs to be delayed further.

UTEX guarantees the identity of the organism as specified in its strain history, but does not quantify the number of organisms or other culture characteristics.