UTEX 625
Synechococcus leopoliensis

Algae Details

UTEX Number: 625
Class: Cyanophyceae
Strain: Synechococcus leopoliensis
Medium: BG-11 Medium (BG-11)
Origin: Austin, Texas, USA
Description of Location: Waller Creek
Type Culture: Yes
Collection: W.A. Kratz
Isolation: M.B. Allen (1952)
Isolator Number: M2.1.1
Deposition: J. Meyers (12/54)
Relatives: CCAP 1405/1; SAG B 1402-1; ATCC 27144 aka ?ATCC 27344 aka IUCC 625 phage host; PCC 6301 S. (cluster 1) elongatus; UTCC 102; IAM M-6; NIBB 1018
Also Known As: Synechococcus sp.; S. elongatus; Anacystis nidulans
Notes: identified by F. Drouet as Anacystis nidulans; deposited as Tx20 Anacystis nidulans (Kratz & Myers 1955); 1970 renamed (Komíçrek 1970); clumping/genetic transfer (Prasatik-Cole & Brand 1983); carbon dioxide/photosynthetic activity (Mayo et al. 1986); fluoride toxicity-resistance (Nichol et al. 1987); glyoxylate cycle, isocitrate lyase, malate synthase (Eley 1988); produces metalothionein (Olafson et al. 1988); photosystem I, polypeptides binding chlorophyll a and B-carotene (Alhadeff et al. 1988); chlorophylide a in plasma membrane (Hinterstoisser et al. 1988); ampicillin-resistant transformants (Lightfoot et al. 1988); heterologous hybridization/E. coli, glutamate synthesis (Lightfoot et al. 1989); 47-kDa, membranes, nitrate transport (Sivak et al. 1989); RuBPCO (Daniell et al. 1989); glycolate excretion (Ronstí_m & Bergman 1989); hydrogen peroxide/light effect (Roncel et al. 1989); ribosomal protein genes/elongation factor genes (Meng et al. 1989); growth and pigmentation (Hauschild et al. 1991); grown in shaded light; rendered axenic by David R. Nobles, Jr. on 02/06/09

General Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.

Preparation of Living Algal Strains For Orders

For agar cultures, strains are transferred to the surface of fresh agar slants in 20 mm diamter glass screw-cap tubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears.

Agar and liquid cultures are packaged within a Styrofoam box and shipped the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

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