UTEX 625
Synechococcus leopoliensis


Condition: Axenic

Medium Type: Agar Slant


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View General Maintenance Conditions


Algae Details

UTEX Number: 625
Class: Cyanophyceae
Strain: Synechococcus leopoliensis
Medium: BG-11 Medium (BG-11)
Origin: Austin, Texas, USA
Description of Location: Waller Creek
GPS:
Type Culture: Yes
Collection: W.A. Kratz
Isolation: M.B. Allen (1952)
Isolator Number: M2.1.1
Deposition: J. Meyers (12/54)
Relatives: CCAP 1405/1; SAG B 1402-1; ATCC 27144 aka ?ATCC 27344 aka IUCC 625 phage host; PCC 6301 S. (cluster 1) elongatus; UTCC 102; IAM M-6; NIBB 1018
Also Known As: Synechococcus sp.; S. elongatus; Anacystis nidulans
Notes: identified by F. Drouet as Anacystis nidulans; deposited as Tx20 Anacystis nidulans (Kratz & Myers 1955); 1970 renamed (Komíçrek 1970); clumping/genetic transfer (Prasatik-Cole & Brand 1983); carbon dioxide/photosynthetic activity (Mayo et al. 1986); fluoride toxicity-resistance (Nichol et al. 1987); glyoxylate cycle, isocitrate lyase, malate synthase (Eley 1988); produces metalothionein (Olafson et al. 1988); photosystem I, polypeptides binding chlorophyll a and B-carotene (Alhadeff et al. 1988); chlorophylide a in plasma membrane (Hinterstoisser et al. 1988); ampicillin-resistant transformants (Lightfoot et al. 1988); heterologous hybridization/E. coli, glutamate synthesis (Lightfoot et al. 1989); 47-kDa, membranes, nitrate transport (Sivak et al. 1989); RuBPCO (Daniell et al. 1989); glycolate excretion (Ronstí_m & Bergman 1989); hydrogen peroxide/light effect (Roncel et al. 1989); ribosomal protein genes/elongation factor genes (Meng et al. 1989); growth and pigmentation (Hauschild et al. 1991); grown in shaded light; rendered axenic by David R. Nobles, Jr. on 02/06/09

General Long-Term Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12:12 Light/Dark Cycle

To reduce the risk of losing newly acquired cultures, it is highly recommended to initially duplicate the Collection's long-term maintenance conditions. Please note that these general maintenance conditions are not designed for optimal algal growth rates or large quantities. For information on the best growth conditions and media, consult other literature or conduct your own experiments. Once a stock culture is established, subcultures can be used to test other conditions.

You can find additional details on the long-term culture maintenance conditions on the Culture Maintenance Guides page.


Preparation of Living Algal Strains For Orders

Agar cultures are transferred to fresh agar slants in 20-mm glass screwcap tubes. After quality control (QC) checks via microscopy are completed, the tubes are cultured under a diurnal light cycle for a minimum of three (3) days.

Cultures are sealed and packaged in a temperature-regulating mailer and shipped the same day.

UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture and only guarantees the identity of the organism as specified in the strain history.


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