UTEX 90
Chlamydomonas reinhardtii

UTEX Prefix Labeling System

LB Xenic strain maintained in liquid media
B Xenic strain maintained on an agar slant
'no prefix' Axenic strain, free of any contaminants and maintained on an agar slant


Algae Detail

UTEX Number: 90
Class: Chlorophyceae
Strain: Chlamydomonas reinhardtii
Media: Soil Extract Medium
Origin: Amherst, Massachusetts, USA
Description of Location: potato field
Type Culture: No
Collection:
Isolation: G.M. Smith (1945)
Isolator Number:
Deposition: CCAP (1952-5)
Relatives: CCAP 11/32A; SAG 11-32b; CGC CC-409; CGC CC-1010; IAM C-239; NIBB 4014
Also Known As: Chlamydomonas reinhardi
Notes: mating type plus [E&S]; [When strains CCAP 11/32a (plus) &11/32b (minus) were introduced to IUCC, they were designated 89 (plus) and 90 (minus) to conform with CCAP. Subsequently, E.I. Friedmann (pers. Comm.) found that 89 = CCAP 11/32b (minus) and 90 = CCAP 11/32a (plus). In addition, Elizabeth Harris (1984, pers. Comm.) equates SAG 11-32b and UTEX 90 with mt+. Thereafter, the cultures related to UTEX 90 are mating type plus] used in combination with UTEX 1648 Selenastrum capricornutum & UTEX 749 Ankistrodesmus falcatus as food for Daphnia & Ceriodaphnia cultures (T. Waller 1988, pers. Comm.); Ca++, galvanotaxis (Nultsch & Dolle 1987); calmodulin, cAMP, gamete flagella (Pasquale & Goodenough 1988); dynein, flagella (Sakakibara & Kamiya 1989); detectable hydrogen photoproduction (Brand et al. 1989); (Ettl 1959, Levine & Ebersold 1960 [incomplete reference]); (Coleman 1982); 7/50 to CCAP from Smith via J. Hartshorne

General Maintenance Conditions

Temperature: 20 °C
Light source: cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.

Preparation of Living Algal Strains

Aliquots of algal strains that are maintained on agar slants are transferred to the surface of fresh slants in 20 mm diameter glass screw-captubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears. Approximately 15 mL aliquots of strains that are maintained as liquid cultures are transferred to screw-cap tubes on the day of shipment. Agar and liquid cultures for a customer are packaged within a Styrofoam™ box and shipped on the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

  • Algae Detail

    UTEX Number: 90
    Class: Chlorophyceae
    Strain: Chlamydomonas reinhardtii
    Media: Soil Extract Medium
    Origin: Amherst, Massachusetts, USA
    Description of Location: potato field
    Type Culture: No
    Collection:
    Isolation: G.M. Smith (1945)
    Isolator Number:
    Deposition: CCAP (1952-5)
    Relatives: CCAP 11/32A; SAG 11-32b; CGC CC-409; CGC CC-1010; IAM C-239; NIBB 4014
    Also Known As: Chlamydomonas reinhardi
    Notes: mating type plus [E&S]; [When strains CCAP 11/32a (plus) &11/32b (minus) were introduced to IUCC, they were designated 89 (plus) and 90 (minus) to conform with CCAP. Subsequently, E.I. Friedmann (pers. Comm.) found that 89 = CCAP 11/32b (minus) and 90 = CCAP 11/32a (plus). In addition, Elizabeth Harris (1984, pers. Comm.) equates SAG 11-32b and UTEX 90 with mt+. Thereafter, the cultures related to UTEX 90 are mating type plus] used in combination with UTEX 1648 Selenastrum capricornutum & UTEX 749 Ankistrodesmus falcatus as food for Daphnia & Ceriodaphnia cultures (T. Waller 1988, pers. Comm.); Ca++, galvanotaxis (Nultsch & Dolle 1987); calmodulin, cAMP, gamete flagella (Pasquale & Goodenough 1988); dynein, flagella (Sakakibara & Kamiya 1989); detectable hydrogen photoproduction (Brand et al. 1989); (Ettl 1959, Levine & Ebersold 1960 [incomplete reference]); (Coleman 1982); 7/50 to CCAP from Smith via J. Hartshorne

  • General Maintenance Conditions

    Temperature: 20 °C
    Light source: cool-white fluorescent lamps
    Intensity: 3200 lux (maximum)
    Periodicity: 12/12 h L/D

    Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.


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