UTEX 89
Chlamydomonas reinhardtii


Condition: Axenic

Medium Type: Agar Slant


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Algae Details

UTEX Number: 89
Class: Chlorophyceae
Strain: Chlamydomonas reinhardtii
Medium: Soil Extract Medium (SE)
Origin: Amherst, Massachusetts, USA
Description of Location: potato field
GPS:
Type Culture: No
Collection:
Isolation: G.M. Smith (1945)
Isolator Number:
Deposition: CCAP (1952-5)
Relatives: CCAP 11/32B; SAG 11-32a; UTCC 84; CGC CC-1009; IAM C-238; NIBB 4013
Also Known As: Chlamydomonas reinhardi
Notes: mating type minus [E&S]; [When strains CCAP 11/32a (plus) & 11/32b (minus) were introduced to IUCC, they were designated 89 (plus) and 90 (minus) to conform with CCAP. Subsequently, E.I. Friedmann (pers. Comm.) found that 89 = CCAP 11/32b (minus) and 90 = CCAP 11/32a (plus). In addition, Elizabeth Harris (1984, pers. Comm.) equates SAG 11-32b and UTEX 90 with mt+. Thereafter, the cultures related to UTEX 89 are mating type minus] lacking fluorescent flagella (Coleman 1988); antibacterial activity (Cannell et al. 1988b); detectable hydrogen photoproduction (Brand et al. 1989); loroxanthin (Fawley 1991); (Ettl 1959, Levine & Ebersold 1960 [incomplete reference]); 7/50 to CCAP from Smith via J. Hartshorne

General Long-Term Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12:12 Light/Dark Cycle

To reduce the risk of losing newly acquired cultures, it is highly recommended to initially duplicate the Collection's long-term maintenance conditions. Please note that these general maintenance conditions are not designed for optimal algal growth rates or large quantities. For information on the best growth conditions and media, consult other literature or conduct your own experiments. Once a stock culture is established, subcultures can be used to test other conditions.

You can find additional details on the long-term culture maintenance conditions on the Culture Maintenance Guides page.


Preparation of Living Algal Strains For Orders

Agar cultures are transferred to fresh agar slants in 20-mm glass screwcap tubes. After quality control (QC) checks via microscopy are completed, the tubes are cultured under a diurnal light cycle for a minimum of three (3) days.

Cultures are sealed and packaged in a temperature-regulating mailer and shipped the same day.

UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture and only guarantees the identity of the organism as specified in the strain history.


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