UTEX sells TAP Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

TAP Medium

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TAP Medium


For 2 L Total
  1. To approximately 1600 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring the total volume to 2 L with dH2O.
    • For 1.5% agar medium add 30 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Allow to cool and store at refrigerator temperature.
    • For agar medium, dispense before agar solidifies.

# Component Amount Stock Solution Concentration Final Concentration
1 Beijerinck's Solution 100 mL/2 L
2 Phosphate Buffer Stock Solution 17 mL/2 L
3 Hunter's Trace Stock Solution 2 mL/2 L
4 Tris Acetate Stock Solution 20 mL/2 L

Important Notes To Consider:

  • The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
  • When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
  • Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.