TAP Medium




TAP Medium Recipe

 

 

Directions

For 2 L Total

  1. To approximately 1600 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring the total volume to 2 L with dH2O.
    • *For 1.5% agar medium add 30 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Allow to cool and store at refrigerator temperature.
    • *For agar medium, dispense before agar solidifies.

# Component Amount Stock Solution Concentration Final Concentration
1 Beijerinck's Solution 100 mL/2 L    
2 Phosphate Buffer Stock Solution 17 mL/2 L    
3 Hunter's Trace Stock Solution 2 mL/2 L    
4 Tris Acetate Stock Solution 20 mL/2 L