For 2 L Total
1. To approximately 1600 mL of dH2O, add each of the components in the order specified while stirring continuously.
2. Bring the total volume to 2 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
4. Cover and autoclave medium.
5. Allow to cool and store at refrigerator temperature.
*For agar medium dispense before agar solidifies.