TAP Medium

TAP Medium Recipe

For 2 L Total

1. To approximately 1600 mL of dH2O, add each of the components in the order specified while stirring continuously.
2. Bring the total volume to 2 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
4. Cover and autoclave medium.
5. Allow to cool and store at refrigerator temperature.
*For agar medium dispense before agar solidifies.

# Component Amount Stock Solution
Final Concentration
1 Beijerinck's Solution 100 mL/2 L    
2 Phosphate Buffer Stock Solution 17 mL/2 L    
3 Hunter's Trace Stock Solution 2 mL/2 L    
4 Tris Acetate Stock Solution 20 mL/2 L