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Tris Acetate Stock Solution Recipe

Directions

For 500 mL Total Volume

  1. To approximately 350 mL of d-H2O, add the following components in the order listed while stirring continuously.
  2. Transfer to a 500-mL graduated cylinder and bring total volume to 500 mL with d-H2O.
# Component Amount Stock Solution Concentration Final Concentration in TAP Medium
1 Trisma Base 121 g/500 mL 2 M 0.02 M
2 Glacial Acetic Acid 50 mL/500 mL 1.75 M 0.017 M

Note: Measure pH to ensure it is above 7.