UTEX sells 5/3 Soil+Seawater Agar Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

5/3 Soil+Seawater Agar Medium

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5/3 Soil+Seawater Agar Medium

*5/3 Soil + Seawater Agar Medium is only available in 10-mL agar tubes*

For marine algae that require salinities at 48 ppt.


For 1 L Total

  1. Filter 1 L of Soil+Seawater Medium to remove soil particles.
  2. Add 15 g of natural sea-salt in a stirring beaker wait until fully dissolved.
  3. Add 15 g of agar into the flask; do not mix.
  4. Cover and autoclave medium.
  5. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Soil+Seawater Medium 1 L
2 Natural sea-salt 15 g/L

Important Notes To Consider:

  • The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
  • When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
  • Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.