UTEX sells 5/3 Soil+Seawater Agar Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.
5/3 Soil+Seawater Agar Medium
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$ 20.00
Deadline to place an order for 5/3 Soil+Seawater Agar Medium with a 2025 shipment date has passed. New orders will not ship until the 2026 new year—see announcement or order confirmation email for your expected shipping date.
5/3 Soil+Seawater Agar Medium
*5/3 Soil + Seawater Agar Medium is only available in 10-mL agar tubes*
For marine algae that require salinities at 48 ppt.
Directions
For 1 L Total
- Filter 1 L of Soil+Seawater Medium to remove soil particles.
- Add 15 g of natural sea-salt in a stirring beaker wait until fully dissolved.
- Add 15 g of agar into the flask; do not mix.
- Cover and autoclave medium.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | Soil+Seawater Medium | 1 L | ||
| 2 | Natural sea-salt | 15 g/L |
Important Notes To Consider:
- The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
- When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
- Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.
Additional algal culture media recipes can be found at the following other major Collections' websites:
- Culture Collection of Algae and Protozoa (CCAP)
- National Center for Marine Algae and Microbiota (NCMA)
- National Institute for Environmental Studies (NIES) Microbial Culture Collection
- The Pasteur Culture Collection of Cyanobacteria (PCC)
- The Culture Collection of Algae at Goettingen University (SAG)
- Canadian Phycological Culture Centre (CPCC)
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