F/2 +NH4 Medium

UTEX sells F/2 +NH4 Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

F/2 +NH₄ Medium

Saltwater Medium | 6 < pH < 8

Micronutrients Natural Seawater NH4 NO3 Silicate Biotin Thiamine Vitamin B12

View Media Recipe

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$ 55.00

Deadline to place an order for F/2 +NH₄ Medium with a 2024 shipment date:
Dec 11, 2024 at 5:00 PM CST

F/2 +NH₄ Medium

Directions

Prepare F/2 Medium.
Dispense 175 mL into 250 mL flasks.
Using the tip of a pasteur pipette, add a pinch of NH₄MgPO₄ to each flask.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	F/2 Medium	1 L
2	NH₄MgPO₄ (Sigma 529354)

F/2 +NH4 Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.