Cyanophycean Medium



Cyanophycean Medium Recipe

Suitable for blue-green algae.

 

Directions

For 1 L Total

  1. To approximately 975 mL of dH2O add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 Liter with dH2O.
    • *For 1.5% agar medium, add 15 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 P-IV Metal Solution 6 mL/L  
2 KNO3 (Baker 3190) 5 g/L   50 mM
3 K2HPO4 (Sigma P 3786) 0.05 g/L   0.29 mM
4 MgSO4·7H2O (Sigma 230391) 0.05 g/L   0.2 mM