Cyanophycean Medium



Cyanophycean Medium Recipe

Directions 
Suitable for blue-green algae.

For 1 L Total

1. To approximately 975 mL of dH2O add each of the components in the order specified while stirring continuously.
2. Bring the total volume to 1 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
3. Cover and autoclave medium.
4. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 P-IV Metal Solution 6 mL/L    
2 KNO3 (Baker 3190) 5 g/L   50 mM
3 K2HPO4 (Sigma P 3786) 0.05 g/L   0.29 mM
4 MgSO4·7H2O (Sigma 230391) 0.05 g/L   0.2 mM