BG-11 + 1% NaCl Medium



BG-11 + 1% NaCl Medium Recipe

Directions 
For 1 L Total

Liquid media:
1. Prepare BG11-1Medium through step one.
2. Add NaCl then bring total volume to 1 L with dH2O.
3. Cover and autoclave medium.
4. Store at refrigerator temperature.

Agar media:
1. Prepare BG11-1Medium through step one.
2. Add NaCl then bring total volume to 500 mL with dH2O.
3. In a separate container add 15 g of agar to 500 mL of dH2O.
4. Cover and autoclave both solutions.
5. In a water bath allow both solutions to cool to 45-50 °C.
6. Add sterile Sodium Thiosulfate to agar solution and mix well.
7. Combine both agar and liquid solutions, mix well. Note the agar can solidify quickly.
8. Allow to cool then store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 BG-11 Medium 1 L    
2 NaCl (Fisher S271-500) 10 g/L   0.17 M