BG-11 + 1% NaCl Medium



BG-11 + 1% NaCl Medium Recipe

 

Directions

For 1 L Total

Liquid media:

  1. Prepare BG-11 Medium through step one.
  2. Add NaCl then bring total volume to 1 L with dH2O.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

Agar media:

  1. Prepare BG-11 Medium through step one.
  2. Add NaCl then bring total volume to 500 mL with dH2O.
  3. In a separate container add 15 g of agar to 500 mL of dH2O.
  4. Cover and autoclave both solutions.
  5. In a water bath allow both solutions to cool to 45-50 °C.
  6. Add sterile Sodium Thiosulfate to agar solution and mix well.
  7. Combine both agar and liquid solutions, mix well. Note the agar can solidify quickly.
  8. Allow to cool then store at refrigerator temperature.
# Component Amount Stock Solution Concentration Final Concentration
1 BG-11 Medium 1 L    
2 NaCl (Fisher S271-500) 10 g/L   0.17 M