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BG-11(-N) Medium is now available in larger volumes.
View the 10-Liter and 100-Liter BG-11(-N) Media Kits.
BG-11(-N) Medium
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$ 55.00
BG-11(-N) Medium
Improved recipe as of March 2009
Directions
For 1 L Total
Liquid media:
- To approximately 900 mL of dH2O add the first 9 components in the order specified while stirring continuously.
- Bring total volume to 1 L with dH2O.
- Cover and autoclave medium.
- Allow to cool then store at refrigerator temperature.
Agar media:
- To approximately 400 mL of dH2O add the first 9 components in the order specified while stirring continuously.
- Bring total volume to 500 mL with dH2O.
- In a separate container add 15 g of agar to 500 mL of dH2O.
- Cover and autoclave both solutions.
- In a water bath allow both solutions to cool to 45-50 °C.
- Add sterile Sodium Thiosulfate (Component 10) to agar solution and mix well.
- Combine both agar and liquid solutions, mix well. Note the agar can solidify quickly.
- Allow to cool then store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | K2HPO4 (CAS: 7758-11-4) |
10 mL/L | 0.8 g/200 mL | 0.22 mM |
| 2 | MgSO4•7H2O (CAS: 10034-99-8) |
10 mL/L | 1.5 g/200 mL | 0.3 mM |
| 3 | CaCl2•2H2O (CAS: 10035-04-8) |
10 mL/L | 0.72 g/200 mL | 0.24 mM |
| 4 | Citric Acid•H2O (CAS: 5949-29-1) |
10 mL/L | 0.12 g/200 mL | 0.012 mM |
| 5 | Ferric Ammonium Citrate (CAS: 1185-57-5) |
10 mL/L | 0.12 g/200 mL | 0.02 mM |
| 6 | Na2EDTA•2H2O (CAS: 6381-92-6) |
10 mL/L | 0.02 g/200 mL | 0.002 mM |
| 7 | Na2CO3 (CAS: 497-19-8) |
10 mL/L | 0.4 g/200 mL | 0.18 mM |
| 8 | BG-11 Trace Metals Solution | 1 mL/L | ||
| 9 | Sodium Thiosulfate Pentahydrate (agar media only,sterile) (CAS: 10102-17-7) |
1 mL/L | 49.6 g/200 mL | 1 mM |
Important Notes To Consider:
- The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
- When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
- Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.
Additional algal culture media recipes can be found at the following other major Collections' websites:
- Culture Collection of Algae and Protozoa (CCAP)
- National Center for Marine Algae and Microbiota (NCMA)
- National Institute for Environmental Studies (NIES) Microbial Culture Collection
- The Pasteur Culture Collection of Cyanobacteria (PCC)
- The Culture Collection of Algae at Goettingen University (SAG)
- Canadian Phycological Culture Centre (CPCC)
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