10L Media Kit: BG-11(-N) Medium
$ 170.00 $ 550.00
Deadline to place an order for 10L Media Kit: BG-11(-N) Medium with a 2024 shipment date has passed. New orders will not ship until the 2025 new year—see announcement or order confirmation email for the expected ship date.
10L Media Kit: BG-11(-N) Medium
This medium does not contain a nitrogen source. BG-11(-N) medium (Allen and Stanier 1968) was derived from medium no. 11 (Hughes et al. 1958) for the culture of freshwater, soil, thermal, and marine cyanobacteria that do not require a source of nitrogen.
Media kit includes all the necessary chemical components to prepare 10 Liters of BG-11(-N) Medium. All UTEX Media Kit solutions are shipped sterile and should be handled aseptically to avoid contamination.
Looking for BG-11(-N) Medium in 1-Liter and test tube volumes?
Medium At A Glance
| Water Type | Freshwater |
| pH of Medium | pH>8 |
| Contains | Micronutrients |
Directions
For 10L Total Volume
- Obtain and wear the appropriate PPE.
- To approximately 9.7 Liters (9,700 mL) of dH2O, add the first three solutions (Components #1-3) in order while stirring.
- Bring total volume to 10 Liters (10,000 mL) with dH2O.
- Cover and autoclave or filter-sterilize if non-sterile water was used.
- When cooled, aseptically add Component #4.
- Store at refrigerator temperatures.
| Components In Kit | Final Concentration |
|---|---|
| MgSO4•7H2O | 0.23 mM |
| CaCl2·2H2O | 0.24 mM |
| Citric Acid·H2O | 0.031 mM |
| BG-11 Trace Metals Solution | |
| Na2EDTA·2H2O | 0.0027 mM |
| Ferric Ammonium Citrate | 0.021 mM |
| K2HPO4 | 0.23 mM |
| Na2CO3 | 0.19 mM |
Important Notes To Consider:
- The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
- When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
- Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.
