UTEX LB 809
Pleodorina californica

UTEX Prefix Labeling System

LB Xenic strain maintained in liquid media
B Xenic strain maintained on an agar slant
'no prefix' Axenic strain, free of any contaminants and maintained on an agar slant


Algae Detail

UTEX Number: 809
Class: Chlorophyceae
Strain: Pleodorina californica
Media: Modified Bold 3N Medium
Origin: Bloomington, Indiana, USA
Description of Location: water
Type Culture: No
Collection:
Isolation: R.C. Starr
Isolator Number:
Deposition:
Relatives: IAM C-336; NIES-735
Also Known As: Eudorina californica
Notes: homothallic, dioecious; taxonomy (Nozaki et al. 1989, 1998b, 2000, 2002a, 2002b, Nozaki & Krienitz 2001); (Goldstein 1964); In Eudorina somatic cells are either lacking or facultative, and in Pleodorina obligate somatic cells are differentiated in all spaeroids (Nozaki et al. 1989)

18s rRNA, partial sequence, NCBI Accession: LC086358

General Maintenance Conditions

Temperature: 20 °C
Light source: cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.

** Estimate for transfer interval based on culturing conditions utilized at UTEX (long-term maintenance).

Preparation of Living Algal Strains

Aliquots of algal strains that are maintained on agar slants are transferred to the surface of fresh slants in 20 mm diameter glass screw-captubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears. Approximately 15 mL aliquots of strains that are maintained as liquid cultures are transferred to screw-cap tubes on the day of shipment. Agar and liquid cultures for a customer are packaged within a Styrofoam™ box and shipped on the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

  • Algae Detail

    UTEX Number: 809
    Class: Chlorophyceae
    Strain: Pleodorina californica
    Media: Modified Bold 3N Medium
    Origin: Bloomington, Indiana, USA
    Description of Location: water
    Type Culture: No
    Collection:
    Isolation: R.C. Starr
    Isolator Number:
    Deposition:
    Relatives: IAM C-336; NIES-735
    Also Known As: Eudorina californica
    Notes: homothallic, dioecious; taxonomy (Nozaki et al. 1989, 1998b, 2000, 2002a, 2002b, Nozaki & Krienitz 2001); (Goldstein 1964); In Eudorina somatic cells are either lacking or facultative, and in Pleodorina obligate somatic cells are differentiated in all spaeroids (Nozaki et al. 1989)

    18s rRNA, partial sequence, NCBI Accession: LC086358

  • General Maintenance Conditions

    Temperature: 20 °C
    Light source: cool-white fluorescent lamps
    Intensity: 3200 lux (maximum)
    Periodicity: 12/12 h L/D

    Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.





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