UTEX B 3239
Oophila amblystomatis


Condition: Xenic

Medium Type: Agar Slant


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View General Maintenance Conditions


Algae Details

UTEX Number: 3239
Class: Chlorophyceae
Strain: Oophila amblystomatis Bishop & Garbary 2024
Medium: Modified Bold 3N Medium (MB3N)
Origin: Michaux State Forest, Pennsylvania, USA
Description of Location: Algae extracted from Rana sylvatica eggs collected maintained in 10 °C incubator with 12/12 light cycle. Algae extracted from intracapsular fluid after sterilizing the outside of the egg capsule with 70% ethanol and rinsing in sterile PBS.
GPS: 39.9385, -77.4588
Type Culture: No
Collection: Elliana Vickers (03/11/2021)
Isolation: Ryan Kerney (03/25/2021)
Isolator Number: RASA_7
Deposition: Ryan Kerney (02/23/2024)
Relatives:
Also Known As:
Notes:

General Long-Term Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12:12 Light/Dark Cycle

To reduce the risk of losing newly acquired cultures, it is highly recommended to initially duplicate the Collection's long-term maintenance conditions. Please note that these general maintenance conditions are not designed for optimal algal growth rates or large quantities. For information on the best growth conditions and media, consult other literature or conduct your own experiments. Once a stock culture is established, subcultures can be used to test other conditions.

You can find additional details on the long-term culture maintenance conditions on the Culture Maintenance Guides page.


Preparation of Living Algal Strains For Orders

Agar cultures are transferred to fresh agar slants in 20-mm glass screwcap tubes. After quality control (QC) checks via microscopy are completed, the tubes are cultured under a diurnal light cycle for a minimum of three (3) days.

Cultures are sealed and packaged in a temperature-regulating mailer and shipped the same day.

UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture and only guarantees the identity of the organism as specified in the strain history.


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