UTEX B 3223
Johansenicoccus eremophilus

Algae Details

UTEX Number: 3223
Class: Chlorophyceae
Strain: Johansenicoccus eremophilus (Fucikova and Pietrasiak)
Medium: Modified Bold 3N Medium (MB3N)
Origin: Cottonwood, CA, USA
Description of Location: Sandy, gravelly soil derived from a granitic outcrop west of Eagle Mountains. The site was vegetated with desert plants typical for the Colorado Desert and also exhibited well developed desert soil crusts. The sample was of the mature soil crust.
GPS: 33.763056, -115.796111
Type Culture: Yes
Collection: Nicole Pietrasiak (06/2006)
Isolation: Nicole Pietrasiak (2006)
Isolator Number: WJT24VFNP31
Deposition: Nicole Pietrasiak (05/15/2023)
Also Known As:
Notes: The material was collected as part of a larger study focused on the biodiversity of JTNP soil crusts (Pietrasiak et al. 2011; Flechtner et al. 2013).

General Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.

Preparation of Living Algal Strains For Orders

For agar cultures, strains are transferred to the surface of fresh agar slants in 20 mm diamter glass screw-cap tubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears.

Agar and liquid cultures are packaged within a Styrofoam box and shipped the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

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