UTEX B 251
Chlorella fusca var. vacuolata

Algae Details

UTEX Number: 251
Class: Chlorophyceae
Strain: Chlorella fusca var. vacuolata
Medium: Proteose Medium (Pr)
Origin: Philadelphia, Pennsylvania, USA
Description of Location: bark
Type Culture: Yes
Isolation: R. Emerson (1923)
Isolator Number:
Deposition: CCAP (1952-5)
Relatives: SAG 211-8b; ATCC 11469 C. sp.; IAM C-104; NIVA CHL 11 C. emersonii var. emersonii; CAUP H 6401 Graesiella vacuolata;
Also Known As: Chlorella vulgaris; Scenedesmus sp.; Chlorella emersonii var. emersonii; C. fusca-vacuolata group; C. pyrenoidosa; Graesiella vacuolata
Notes: (Shihira & Krauss 1965); used in Emerson's work on iron deficiency and photosynthesis (George 1976); aka Chlorella fusca-vacuolata group (Komov et al. 1988); heavy metal binding complexes (Gekeler et al. 1988); cell wall carbohydrates, chemotaxonomy (Takeda 1988b); chemotaxonomy, transport of glucose, arginine & proline (Komov et al. 1988); immunocytochemical localization of RuBisCO in pyrenoid (McKay & Gibbs 1989); physiology/biochemistry/taxonomy (Kessler & Huss 1992); The names of the Chlorella strains given in the last list of cultures (Starr & Zeikus 1987) were based primarily upon the taxonomic treatments of Kessler & Maifarth (1960), Kessler & Soeder (1962), Fott & Novakova (1969) and Kessler (1976). In 1992, Kessler & Huss revised the names of axenic UTEX cultures. Subsequently, most of the changes, but not all, were accepted and incorporated in the UTEX list. "[K&H]" tags the 1992 identifications of Kessler & Huss.

Strains that are maintained only in cryopreservation (designated 'cryo only') require a minimum of 4-weeks to grow out before shipment.

Cryopreservation Conditions

Temperature: -190 °C
Storage: Vapor phase of liquid nitrogen dewars

Cryopreservation stabilizes genomic integrity, preserves culture quality, minimizes maintenance costs and reduces the risk of catastrophic loss. A broad variety of techniques employed at the CCAP, at UTEX, and elsewhere, can be found in Day, J.G. and Brand, J.J. Cryopreservation Methods for Maintaining Microalgal Cultures, in Algal Culturing Techniques, ed. R. A. Andersen, Elsevier, Amsterdam, 2006. A relatively simple method that uses a minimum of specialized equipment, yet allows a large variety of microalgae to be cryopreserved with high viability, can be found on the Cryopreservation of Microalgae page.

Preparation of Strains Maintained Only In Cryopreservation For Orders

Strains that are maintained only in cryopreservation (cryo only) require a minimum of 4-weeks to thaw, grow out, and check viability before shipment. An estimated shipping date will be emailed when purchased. Updates via email will be sent if the strain is ready sooner or needs to be delayed further.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

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