UTEX 2229
Chlorella kessleri

View Algae Details

View General Maintenance Conditions

Algae Details

UTEX Number: 2229
Class: Chlorophyceae
Strain: Chlorella kessleri
Medium: Modified Bold 3N Medium (MB3N)
Description of Location:
Type Culture: No
Isolation: S. Trelease
Isolator Number: X-ray induced chlorophyll mutant from UTEX 2228
Deposition: S. Beale (2/5/80)
Also Known As: Chlorella vulgaris (Starr & Zeikus 1987)
Notes: deposited as 610; to Beale from S. Granick; grows vigorously on D-glucose in the dark and minerals only in the light; requires light for chlorophyll formation; in the dark, only tiny amounts of chlorophyll and protochlorophyll(ide) are formed; in the light, it is phenotypically identical to the wild type (Herron & Mauzerall 1972); physiology/biochemistry/taxonomy (Kessler & Huss 1992); The names of the Chlorella strains given in the last list of cultures (Starr & Zeikus 1987) were based primarily upon the taxonomic treatments of Kessler & Maifarth (1960), Kessler & Soeder (1962), Fott & Novakova (1969) and Kessler (1976). In 1992, Kessler & Huss revised the names of axenic UTEX cultures. Subsequently, most of the changes, but not all, were accepted and incorporated in the UTEX list. "[K&H]" tags the 1992 identifications of Kessler & Huss.

General Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.

Preparation of Living Algal Strains For Orders

For agar cultures, strains are transferred to the surface of fresh agar slants in 20 mm diamter glass screw-cap tubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears.

Agar and liquid cultures are packaged within a Styrofoam box and shipped the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

Related Items