UTEX 969
Chlamydomonas applanata

UTEX Prefix Labeling System

LB Xenic strain maintained in liquid media
B Xenic strain maintained on an agar slant
'no prefix' Axenic strain, free of any contaminants and maintained on an agar slant


Algae Detail

UTEX Number: 969
Class: Chlorophyceae
Strain: Chlamydomonas applanata
Media: Modified Bold 3N Medium
Origin: Williamson Co., Texas, USA
Description of Location: sandy soil
Type Culture: Yes
Collection:
Isolation: T.R. Deason
Isolator Number: homothallic T-1-12 Chlamydomonas aggregata
Deposition: (7/60)
Relatives: SAG 2.72; IAM C-214
Also Known As: formerly Chlamydomonas aggregata (Starr & Zeikus 1987)
Notes: (Deason & Bold 1960); no detectable hydrogen photoproduction (Brand et al. 1989); In 1990, UTEX, CCAP, and SAG strains of Chlamydomonas were investigated by H. Ettl & U.G. Schlosser and many were found to be incorrectly named. Subsequently, most of the changes, but not all, were accepted and incorporated in the UTEX list. "[E&S]" tags the 1990 identifications of Ettl & Schlosser (pers. comm.).

General Maintenance Conditions

Temperature: 20 °C
Light source: cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.

** Estimate for transfer interval based on culturing conditions utilized at UTEX (long-term maintenance).

Preparation of Living Algal Strains

Aliquots of algal strains that are maintained on agar slants are transferred to the surface of fresh slants in 20 mm diameter glass screw-captubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears. Approximately 15 mL aliquots of strains that are maintained as liquid cultures are transferred to screw-cap tubes on the day of shipment. Agar and liquid cultures for a customer are packaged within a Styrofoam™ box and shipped on the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

  • Algae Detail

    UTEX Number: 969
    Class: Chlorophyceae
    Strain: Chlamydomonas applanata
    Media: Modified Bold 3N Medium
    Origin: Williamson Co., Texas, USA
    Description of Location: sandy soil
    Type Culture: Yes
    Collection:
    Isolation: T.R. Deason
    Isolator Number: homothallic T-1-12 Chlamydomonas aggregata
    Deposition: (7/60)
    Relatives: SAG 2.72; IAM C-214
    Also Known As: formerly Chlamydomonas aggregata (Starr & Zeikus 1987)
    Notes: (Deason & Bold 1960); no detectable hydrogen photoproduction (Brand et al. 1989); In 1990, UTEX, CCAP, and SAG strains of Chlamydomonas were investigated by H. Ettl & U.G. Schlosser and many were found to be incorrectly named. Subsequently, most of the changes, but not all, were accepted and incorporated in the UTEX list. "[E&S]" tags the 1990 identifications of Ettl & Schlosser (pers. comm.).

  • General Maintenance Conditions

    Temperature: 20 °C
    Light source: cool-white fluorescent lamps
    Intensity: 3200 lux (maximum)
    Periodicity: 12/12 h L/D

    Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.


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