UTEX 260
Chlorella sorokiniana

UTEX Prefix Labeling System

LB Xenic strain maintained in liquid media
B Xenic strain maintained on an agar slant
'no prefix' Axenic strain, free of any contaminants and maintained on an agar slant


Algae Detail

UTEX Number: 260
Class: Chlorophyceae
Strain: Chlorella sorokiniana
Media: Proteose Medium
Origin:
Description of Location:
Type Culture: Yes
Collection:
Isolation: E.G. Pringsheim (before 1929)
Isolator Number: C. pyrenoidosa
Deposition: CCAP (1952-5)
Relatives: SAG 211-11c; CCAP 211/11c [dec.] C. vulgaris var. vulgaris aka. Stamm 3 aka Meyer 3
Also Known As: formerly C. vulgaris (Starr & Zeikus 1987); C. candida (Shihira & Krauss 1965); C. pyrenoidosa; C. vulgaris var. vulgaris
Notes: physiology/biochemistry/taxonomy (Kessler & Huss 1992); The names of the Chlorella strains given in the last list of cultures (Starr & Zeikus 1987) were based primarily upon the taxonomic treatments of Kessler & Maifarth (1960), Kessler & Soeder (1962), Fott & Novakova (1969) and Kessler (1976). In 1992, Kessler & Huss revised the names of axenic UTEX cultures. Subsequently, most of the changes, but not all, were accepted and incorporated in the UTEX list. "[K&H]" tags the 1992 identifications of Kessler & Huss.

General Maintenance Conditions

Temperature: 20 °C
Light source: cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.

** Estimate for transfer interval based on culturing conditions utilized at UTEX (long-term maintenance).

Preparation of Living Algal Strains

Aliquots of algal strains that are maintained on agar slants are transferred to the surface of fresh slants in 20 mm diameter glass screw-captubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears. Approximately 15 mL aliquots of strains that are maintained as liquid cultures are transferred to screw-cap tubes on the day of shipment. Agar and liquid cultures for a customer are packaged within a Styrofoam™ box and shipped on the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.

  • Algae Detail

    UTEX Number: 260
    Class: Chlorophyceae
    Strain: Chlorella sorokiniana
    Media: Proteose Medium
    Origin:
    Description of Location:
    Type Culture: Yes
    Collection:
    Isolation: E.G. Pringsheim (before 1929)
    Isolator Number: C. pyrenoidosa
    Deposition: CCAP (1952-5)
    Relatives: SAG 211-11c; CCAP 211/11c [dec.] C. vulgaris var. vulgaris aka. Stamm 3 aka Meyer 3
    Also Known As: formerly C. vulgaris (Starr & Zeikus 1987); C. candida (Shihira & Krauss 1965); C. pyrenoidosa; C. vulgaris var. vulgaris
    Notes: physiology/biochemistry/taxonomy (Kessler & Huss 1992); The names of the Chlorella strains given in the last list of cultures (Starr & Zeikus 1987) were based primarily upon the taxonomic treatments of Kessler & Maifarth (1960), Kessler & Soeder (1962), Fott & Novakova (1969) and Kessler (1976). In 1992, Kessler & Huss revised the names of axenic UTEX cultures. Subsequently, most of the changes, but not all, were accepted and incorporated in the UTEX list. "[K&H]" tags the 1992 identifications of Kessler & Huss.

  • General Maintenance Conditions

    Temperature: 20 °C
    Light source: cool-white fluorescent lamps
    Intensity: 3200 lux (maximum)
    Periodicity: 12/12 h L/D

    Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.


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