UTEX 252
Chlorella fusca var. vacuolata


Condition: Axenic

Medium Type: Agar Slant


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View General Maintenance Conditions


Algae Details

UTEX Number: 252
Class: Chlorophyceae
Strain: Chlorella fusca var. vacuolata
Media: Proteose Medium
Origin: Berlin, Germany
Description of Location: tap-water in O. Warburg's lab
Type Culture: No
Collection:
Isolation: R. Emerson (1926)
Isolator Number:
Deposition: CCAP
Relatives: CCAP 211/8C; SAG 211-8c; UTCC 89; NIBB 4001, NIBB 4002, NIBB 4003, NIBB 4004, NIBB 4005, NIBB 4006; IAM C-105
Also Known As: Scenedesmus sp.; Chlorella emersonii var. emersonii; Chlorella emersonii var. globosa; C. pyrenoidosa
Notes: 1946 to CCAP from Emerson; photosystem I/respiration (Sargent & Taylor 1972); cell wall carbohydrates, chemotaxonomy (Takeda 1988b); antibacterial activity (Cannell et al. 1988b); detectable H2 photoproduction (Brand et al. 1989); physiology/biochemistry/taxonomy (Kessler & Huss 1992); The names of the Chlorella strains given in the last list of cultures (Starr & Zeikus 1987) were based primarily upon the taxonomic treatments of Kessler & Maifarth (1960), Kessler & Soeder (1962), Fott & Novakova (1969) and Kessler (1976). In 1992, Kessler & Huss revised the names of axenic UTEX cultures. Subsequently, most of the changes, but not all, were accepted and incorporated in the UTEX list. "[K&H]" tags the 1992 identifications of Kessler & Huss.

General Long-Term Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12:12 Light/Dark Cycle

To reduce the risk of losing newly acquired cultures, it is highly recommended to initially duplicate the Collection's long-term maintenance conditions. Please note that these general maintenance conditions are not designed for optimal algal growth rates or large quantities. For information on the best growth conditions and media, consult other literature or conduct your own experiments. Once a stock culture is established, subcultures can be used to test other conditions.

You can find additional details on the long-term culture maintenance conditions on the Culture Maintenance Guides page.


Preparation of Living Algal Strains For Orders

Agar cultures are transferred to fresh agar slants in 20-mm glass screwcap tubes. After quality control (QC) checks via microscopy are completed, the tubes are cultured under a diurnal light cycle for a minimum of three (3) days.

Cultures are sealed and packaged in a temperature-regulating mailer and shipped the same day.

UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture and only guarantees the identity of the organism as specified in the strain history.


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