UTEX 26
Chlorella vulgaris


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View General Maintenance Conditions

New Green Deal:

As part of the effort to combat the spread of COVID-19, The University of Texas at Austin has implemented a mandatory, campus-wide reduction of laboratory activities to essential operations. In keeping with this mandate, there will be a temporary pause in the distribution of all products from the UTEX Culture Collection of Algae. During this pause, UTEX products will be available for advance purchase at a discounted rate  no discount code required. All orders will be scheduled for delivery when we resume normal operations.



Algae Details

UTEX Number: 26
Class: Chlorophyceae
Strain: Chlorella vulgaris
Medium: Proteose Medium (Pr)
Origin: Czechoslovakia
Description of Location:
GPS:
Type Culture: Yes
Collection:
Isolation: E.G. Pringsheim
Isolator Number: Chlorella vulgaris 1
Deposition: CCAP (1952-5)
Relatives: CCAP 211/8A C. emersonii aka Meyer 10 aka C. emersonii var. emersonii; ATCC 30582 C. sp.; UTCC LB 36
Also Known As: formerly Chlorella pyrenoidosa (Starr & Zeikus 1987); C. photophila (Shihira & Krauss 1965); C. emersonii; C. fusca var. vacuolata
Notes: fluoride toxicity (Nichol et al. 1987); growing freshwater rotifer Brachionus calyciflorus (Bennett & Boraas 1988); photosynthesis/acid pH shock (Nalewajko & O'Mahoney 1989); physiology/biochemistry/taxonomy (Kessler & Huss 1992); (Winokur 1948); The names of the Chlorella strains given in the last list of cultures (Starr & Zeikus 1987) were based primarily upon the taxonomic treatments of Kessler & Maifarth (1960), Kessler & Soeder (1962), Fott & Novakova (1969) and Kessler (1976). In 1992, Kessler & Huss revised the names of axenic UTEX cultures. Subsequently, most of the changes, but not all, were accepted and incorporated in the UTEX list. "[K&H]" tags the 1992 identifications of Kessler & Huss.

General Maintenance Conditions

Temperature: 20 °C
Light source: mix of warm-white & cool-white fluorescent lamps
Intensity: 3200 lux (maximum)
Periodicity: 12/12 h L/D

Those who receive cultures are encouraged to duplicate within reason the conditions used in the Collection, when handling newly acquired cultures, to reduce the chance of losing the culture. After a stock culture is established, subcultures may be used for testing other conditions. These general maintenance conditions are not our recommendations to achieve optimal growth rates and large quantities of algae. Information on the best growth conditions and media must be acquired in other literature or through careful experimentation. For additional details on the long-term culture maintenance conditions utilized at UTEX, visit the Culture Maintenance Guides page.


Preparation of Living Algal Strains For Orders

For agar cultures, strains are transferred to the surface of fresh agar slants in 20 mm diamter glass screw-cap tubes in preparation for shipment. The fresh inocula are grown in a diurnal light cycle for at least 3 days until a macroscopically visible lawn appears.

Agar and liquid cultures are packaged within a Styrofoam box and shipped the same day they are packaged.

One tube of agar or liquid culture constitutes an order for a single culture of a single strain of living algae. UTEX makes no attempt to quantify the number of organisms or other characteristics of the culture. UTEX only guarantees the identity of the organism as specified in the strain history.


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