2/3 Enriched Seawater Medium

UTEX sells 2/3 Enriched Seawater Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

2/3 Enriched Seawater Medium

Micronutrients Natural Seawater NO3 Biotin Thiamine Vitamin B12

2/3 Enriched Seawater Medium

For marine algae that require salinities at 20 ppt, the basic Enriched Seawater Medium is combined with sterile distilled water prior to inoculating the new culture, such that 2/3 Enriched Seawater Medium combines Enriched Seawater Medium and sterile distilled water 2:1.

Directions

For 1 L Total

Combine components.
Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	Enriched Seawater Medium	665 mL
2	sterile dH₂O	335 mL

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2/3 Enriched Seawater Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.