Soilwater: VT Medium
Soil having little or no organic content is substituted for garden-type soil; lacks supplements; for some strains of Closterium, freshwater dinoflagellates and others.
For 200 mL Total
- Combine components.
- Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
- Refrigerate 24 hours or more and bring to room temperature before use.
||Stock Solution Concentration