Soilwater: VT Medium

Soilwater: VT Medium Recipe

Soil having little or no organic content is substituted for garden-type soil; lacks supplements; for some strains of Closterium, freshwater dinoflagellates and others.


For 200 mL Total

  1. Combine components.
  2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
  3. Refrigerate 24 hours or more and bring to room temperature before use.

# Component Amount Stock Solution Concentration Final Concentration
1 Vermont Soil 1 tsp
2 dH2O 200 mL