Soilwater: PEA Medium
This basic garden-type soilwater, to which a garden pea is added before steaming, is suitable for some euglenoids, xenic cultures of Astrephomene and other mixotrophic algae.
Directions
For 200 mL Total
- Combine components.
- Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
- Refrigerate 24 hours or more and bring to room temperature before use.
# |
Component |
Amount |
Stock Solution Concentration |
Final Concentration |
1 |
Green House Soil |
1 tsp |
|
|
2 |
Pea (add after dH2O) |
1 per 200 mL |
|
|
3 |
CaCO3 (Fisher C 64) |
1 mg |
|
0.05 mM |
4 |
dH2O |
200 mL |
|
|