Soilwater: GR+/NH4 Medium

UTEX sells Soilwater: GR+/NH4 Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

Soilwater: GR+/NH₄ Medium

Freshwater Medium

NH4 Soil

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$ 55.00

Deadline to place an order for Soilwater: GR+/NH₄ Medium with a 2024 shipment date:
Dec 11, 2024 at 5:00 PM CST

Soilwater: GR+/NH4 Medium

The basic garden-type soilwater; includes a pinch of CaCO₃, which is added to the soil and water after steaming; suitable for most phototrophic freshwater algae. Optional ingredients: add Vitamin B₁₂ to cultures of Volvox; a pinch of NH₄MgPO₄•6H2O added to soilwater cultures of Botryococcus, Synechococcus and some Euglenoids enhances growth and to LB 826 Gonium pectorale increases the numbers of 16-celled colonies formed.

Directions

For 200 mL Total

Prepare Soilwater: GR+ Medium
Add pinch of NH₄MgPO4 after pasteurizing.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	Soilwater: GR+ Medium	200 mL
2	NH₄MgPO₄ (Sigma 529354)	1 mg		0.04 mM

Soilwater: GR+/NH4 Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.