Soilwater: GR- Medium



Soilwater: GR- Medium Recipe

A garden-type soilwater without supplements. Suitable for Spirogyra, Zygnema and a few other phototrophic algae.

Directions

For 200 mL Total (pH 7.3)

  1. Combine components.
  2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
  3. Refrigerate 24 hours or more and bring to room temperature before use.

# Component Amount Stock Solution Concentration Final Concentration
1 Green House Soil 1 tsp
2 dH2O 200 mL