Soilwater: BAR Medium

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Soilwater: BAR Medium Recipe

Suitable for some mixotrophic and most heterotrophic (colorless) euglenoids.

Directions

For 200 mL Total

  1. Combine components.
  2. Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
  3. Refrigerate 24 hours or more and bring to room temperature before use.

# Component Amount Stock Solution Concentration Final Concentration
1 Green House Soil 1 tsp
2 Barley grains (add after dH2O) 3 grains
3 CaCO3 (optional) (Fisher C 64) 1 mg 0.05 mM
4 dH2O 200 mL