Snow Algae Medium

Upcoming Order Deadlines:

Orders for algal culture media placed after 5:00 PM CST on Wednesday, November 14th through Wednesday, November 28th, will not ship until Monday, December 3rd, 2018. There will be no outgoing shipments on November 26th, 2018. Please view more information here.




Snow Algae Medium Recipe

Suitable for snow algae collection.

 

Directions

For 1 L Total

  1. To approximately 500 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O, pH is 5.1 - 5.3
    • *For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 CaCl2·2H2O (Sigma C-3881) 1 mL/L 0.5 g/100 mL dH2O 0.03 mM
2 NaNO3 (Fisher BP360-500) 1 mL/L 2.5 g/100 mL dH2O 0.29 mM
3 NH4Cl (Fisher A 649-500) 1 mL/L 0.5 g/100 mL dH2O 0.093 mM
4 CaSO4.2H2O (Mallinckroft 4300) 1 mL/L 0.4 g/100 mL dH2O 0.023 mM
5 MgSO4·7H2O (Sigma 230391) 1 mL/L 0.5 g/100 mL dH2O 0.02 mM
6 Na2SiO3·9H2O (Sigma 307815) 1 mL/L 0.2 g/100 mL dH2O 0.007 mM
7 FE EDTA 250 mL/L    
8 Minor Nutrients 10 mL/L    
9 K2HPO4 (Sigma P 3786) 2 mL 3.48 g/100 mL dH2O 0.4 mM
10 KH2PO4 (Sigma P 0662) 98 mL 2.72 g/100 mL dH2O 0.02 M