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Snow Algae Medium



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Snow Algae Medium

Suitable for snow algae collection.

Directions

For 1 L Total
  1. To approximately 500 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring the total volume to 1 L with dH2O, pH is 5.1-5.3.
    • For 1.5% agar medium add 15 g of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 CaCl22H2O
(Sigma C-3881)
1 mL/L 0.5 g/100 mL dH2O 0.03 mM
2 NaNO3
(Fisher BP360-500)
1 mL/L 2.5 g/100 mL dH2O 0.29 mM
3 NH4Cl
(Fisher A 649-500)
1 mL/L 0.5 g/100 mL dH2O 0.093 mM
4 CaSO42H2O
(Mallinckroft 4300)
1 mL/L 0.4 g/100 mL dH2O 0.023 mM
5 MgSO47H2O
(Sigma 230391)
1 mL/L 0.5 g/100 mL dH2O 0.02 mM
6 Na2SiO39H2O
(Sigma 307815)
1 mL/L 0.2 g/100 mL dH2O 0.007 mM
7 FE EDTA 250 mL/L
8 Minor Nutrients 10 mL/L
9 K2HPO4
(Sigma P 3786)
2 mL 3.48 g/100 mL dH2O 0.4 mM
10 KH2PO4
(Sigma P 0662)
98 mL 2.72 g/100 mL dH2O 0.02 M

Important Notes To Consider:

  • The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater (and natural freshwater) should be obtained from a non-polluted source.
  • When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
  • Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.