Snow Algae Medium

UTEX sells Snow Algae Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

Snow Algae Medium

Freshwater Medium | pH<6

Micronutrients NH4 NO3

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$ 55.00

Snow Algae Medium

Suitable for snow algae collection.

Directions

For 1 L Total

To approximately 500 mL of dH₂O, add each of the components in the order specified while stirring continuously.
Bring the total volume to 1 L with dH₂O, pH is 5.1-5.3.
- For 1.5% agar medium add 15 g of agar into the flask; do not mix.
Cover and autoclave medium.
Store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	CaCl₂•2H₂O (Sigma C-3881)	1 mL/L	0.5 g/100 mL dH₂O	0.03 mM
2	NaNO₃ (Fisher BP360-500)	1 mL/L	2.5 g/100 mL dH₂O	0.29 mM
3	NH₄Cl (Fisher A 649-500)	1 mL/L	0.5 g/100 mL dH₂O	0.093 mM
4	CaSO₄•2H₂O (Mallinckroft 4300)	1 mL/L	0.4 g/100 mL dH₂O	0.023 mM
5	MgSO₄•7H₂O (Sigma 230391)	1 mL/L	0.5 g/100 mL dH₂O	0.02 mM
6	Na₂SiO₃•9H₂O (Sigma 307815)	1 mL/L	0.2 g/100 mL dH₂O	0.007 mM
7	FE EDTA	250 mL/L
8	Minor Nutrients	10 mL/L
9	K₂HPO₄ (Sigma P 3786)	2 mL	3.48 g/100 mL dH₂O	0.4 mM
10	KH₂PO₄ (Sigma P 0662)	98 mL	2.72 g/100 mL dH₂O	0.02 M

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Snow Algae Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.