Snow Algae Medium



Snow Algae Media Recipe

Directions 

Suitable for snow algae collection.

 

For 1 L Total
1. To approximately 500 mL of dH2O, add each of the components in the order specified while stirring continuously.
2. Bring the total volume to 1 L with dH2O, pH is 5.1-5.3.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
3. Cover and autoclave medium.
4. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 CaCl2·2H2O (Sigma C-3881) 1 mL/L 0.5 g/100 mL dH20 0.03 mM
2 NaNO3 (Fisher BP360-500) 1 mL/L 2.5 g/100 mL dH20 0.29 mM
3 NH4Cl (Fisher A 649-500) 1 mL/L 0.5 g/100 mL dH20 0.093 mM
4 CaSO4.2H2O (Mallinckroft 4300) 1 mL/L 0.4 g/100 mL dH20 0.023 mM
5 MgSO4·7H2O (Sigma 230391) 1 mL/L 0.5 g/100 mL dH20 0.02 mM
6 Na2SiO3·9H2O (Sigma 307815) 1 mL/L 0.2 g/100 mL dH20 0.007 mM
7 FE EDTA 250 mL/L    
8 Minor Nutrients 10 mL/L    
9 K2HPO4 (Sigma P 3786) 2 mL 3.48 g/100 mL dH20 0.4 mM
10 KH2PO4 (Sigma P 0662) 98 mL 2.72 g/100 mL dH20 0.02 M