Porphryridium Medium



Porphyridium Medium Recipe

Directions 
Suitable for axenic marine cultures of Porphyridium, Brachiomonas, Cylindrotheca and others.

For 1 L Total

1. To approximately 350 mL of dH2O, add each of the components in the order specified while stirring continuously.
2. Bring the total volume to 1 L with dH2O.
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
3. Cover and autoclave medium.
4. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 Seawater
(non-sterilized)
500 mL/L    
2 Soilwater: GR+ Medium 100 mL/L    
3 Yeast extract
(Bacto, Difco)
1 g/L    
4 Tryptone (Sigma T 9410)) 1 g/L