Porphryridium Medium



Porphyridium Medium Recipe

Suitable for axenic marine cultures of Porphyridium, Brachiomonas, Cylindrotheca and others.

 

Directions

For 1 L Total

  1. To approximately 350 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring the total volume to 1 L with dH2O.
    • *For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Seawater (non-sterilized) 500 mL/L    
2 Soilwater: GR+ Medium 100 mL/L    
3 Yeast extract (Bacto, Difco) 1 g/L    
4 Tryptone (Sigma T 9410) 1 g/L