Polytomella Medium



Polytomella Medium Recipe

Directions 
Suitable for all axenic cultures of Polytoma, Polytomella and Astasia, the colorless strains of Euglena and 2016 Scenedesmus obliquus.

For 1 L Total

1. To approximately 950 mL of dH2O add each of the components in the order specified while stirring continuously.
2. Bring total volume to 1 L with dH2O .
*For 1.5% agar medium add 15 g of agar into the flask; do not mix.
3. Cover and autoclave medium.
4. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 Yeast extract
(Bacto, Difco)
1 g/L    
2 Tryptone (Sigma T 9410)) 1 g/L    
3 Sodium acetate (Fisher BP 333) 2 g/L   24.4 mM