Polytomella Medium




Polytomella Medium Recipe

Suitable for all axenic cultures of Polytoma, Polytomella and Astasia, the colorless strains of Euglena and 2016 Scenedesmus obliquus.

 

Directions

For 1 L Total

  1. To approximately 950 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O.
    • *For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Yeast extract (Bacto, Difco) 1 g/L    
2 Tryptone (Sigma T 9410) 1 g/L    
3 Sodium acetate (Fisher BP 333) 2 g/L   24.4 mM