Polytomella Medium

UTEX sells Polytomella Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

Polytomella Medium

Freshwater Medium

Organic N Organic Supplements

View Media Recipe

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$ 55.00

Deadline to place an order for Polytomella Medium with a 2024 shipment date:
Dec 11, 2024 at 5:00 PM CST

Polytomella Medium

Suitable for all axenic cultures of Polytoma, Polytomella and Astasia, the colorless strains of Euglena and 2016 Scenedesmus obliquus.

Directions

For 1 L Total

To approximately 950 mL of dH₂O, add each of the components in the order specified while stirring continuously.
Bring total volume to 1 L with dH₂O.
- For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
Cover and autoclave medium.
Store at refrigerator temperature.

#	Component	Amount	Final Concentration
1	Yeast extract (Bacto, Difco)	1 g/L
2	Tryptone (Sigma T 9410)	1 g/L
3	Sodium acetate (Fisher BP 333)	2 g/L	24.4 mM

Polytomella Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.