Polytomella Medium

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Orders for algal culture media placed after 5:00 PM CST on Wednesday, November 14th through Wednesday, November 28th, will not ship until Monday, December 3rd, 2018. There will be no outgoing shipments on November 26th, 2018. Please view more information here.




Polytomella Medium Recipe

Suitable for all axenic cultures of Polytoma, Polytomella and Astasia, the colorless strains of Euglena and 2016 Scenedesmus obliquus.

 

Directions

For 1 L Total

  1. To approximately 950 mL of dH2O, add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O.
    • *For 1.5% agar medium add 15 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Yeast extract (Bacto, Difco) 1 g/L    
2 Tryptone (Sigma T 9410) 1 g/L    
3 Sodium acetate (Fisher BP 333) 2 g/L   24.4 mM