1/3 Soil+Seawater Medium

UTEX sells 1/3 Soil+Seawater Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

1/3 Soil+Seawater Medium

Natural Seawater Soil

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$ 55.00

Deadline to place an order for 1/3 Soil+Seawater Medium with a 2024 shipment date:
Dec 11, 2024 at 5:00 PM CST

1/3 Soil+Seawater Medium

For some marine and brackish-water algae requiring salinities at 10 ppt, glass-distilled water and pasteurized, filtered seawater are combined prior to steaming, such that 1/3 Soil+Seawater contains seawater combined with distilled water 1:2.

Directions

For 200 mL Total

Combine all components.
Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
Refrigerate 24 hours or more and bring to room temperature before using.

#	Component	Amount	Final Concentration
1	Green House Soil	1 tsp
2	CaCO₃ (optional) (Fisher C 64)	1 mg	0.05 mM
3	Pasteurized Seawater	67 mL
4	dH₂O	133 mL

1/3 Soil+Seawater Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.