1/3 Soil+Seawater Medium
For some marine and brackish-water algae requiring salinities at 10 ppt, glass-distilled water and pasteurized, filtered seawater are combined prior to steaming, such that 1/3 Soil+Seawater contains seawater combined with distilled water 1:2.
For 200 mL Total
- Combine all components.
- Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95¡C in 15 minutes. Then increased just over 98¡C for the 3 hour duration. Cooling occurs gradually at room temperature.
- Refrigerate 24 hours or more and bring to room temperature before using.
||Stock Solution Concentration
||Green House Soil
||CaCO3 (optional) (Fisher C 64)