The final medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps.
For 1 L Total
Liquid media: 1. To approximately 900 mL of dH2O add the first 7 components in the order specified while stirring continuously. 2. Bring total volume to 1 L with dH2O. 3. Cover and autoclave medium. 4. Allow to cool. 5. Add 1 sterile barley grain per test tube then store at refrigerator temperature.