Modified Desmidiacean Medium

UTEX sells Modified Desmidiacean Medium at 1X concentration only. Stock solutions and individual components for this specific medium are currently not available for purchase.

Modified Desmidiacean Medium

Freshwater Medium

Barley grains Micronutrients NH4 NO3 Organic Supplements

View Media Recipe

View Available Downloads

$ 55.00

Deadline to place an order for Modified Desmidiacean Medium with a 2024 shipment date:
Dec 11, 2024 at 5:00 PM CST

Modified Desmidiacean Medium

The final medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps.

Directions

For 1 L Total

To approximately 900 mL of dH₂O add the first 7 components in the order specified while stirring continuously.
Bring total volume to 1 L with dH₂O.
Cover and autoclave medium.
Allow to cool.
Add 1 sterile barley grain per test tube then store at refrigerator temperature.

#	Component	Amount	Stock Solution Concentration	Final Concentration
1	KNO₃ (Baker 3190)	10 mL/L	1 g/100 mL	0.9 mM
2	(NH₄)₂HPO₄ (Fisher A686)	5 mL/L	0.2 g/100 mL	0.075 mM
3	MgSO₄•7H₂O (Sigma 230391)	10 mL/L	0.1 g/100 mL	3.3 µM
4	CaSO₄•2H₂O saturated solution (Mallinckroft 4300)	10 mL/L
5	Soilwater: GR+ Medium	20 mL/L
6	Soilwater: Peat Medium	10 mL/L
7	MWC Metal Solution	5 mL/L
8	Barley grains autoclaved	1 grain/10 mL

Modified Desmidiacean Medium Recipe PDF

Safety Data Sheet (SDS)

FAQs:Algal Culture Media

Important Notes To Consider:

The quality of water, including natural seawater, is important. The term 'dH₂O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater and natural freshwater should be obtained from a non-polluted source.
When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.