Modified Desmidiacean Medium



Modified Desmidiacean Medium Recipe

Directions 
The final medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps.

For 1 L Total

Liquid media:
1. To approximately 900 mL of dH2O add the first 7 components in the order specified while stirring continuously.
2. Bring total volume to 1 L with dH2O.
3. Cover and autoclave medium.
4. Allow to cool.
5. Add 1 sterile barley grain per test tube then store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 KNO3 (Baker 3190) 10 mL/L 1 g/100 mL 0.9 mM
2 (NH4)2HPO4 (Fisher A686) 5 mL/L 0.2 g/100 mL 0.075 mM
3 MgSO4·7H2O (Sigma 230391) 10 mL/L 0.1 g/100 mL 3.3 µM
4 CaSO4.2H2O saturated solution (Mallinckroft 4300) 10 mL/L    
5 Soilwater: GR+ Medium 20 mL/L    
6 Soilwater: Peat Medium 10 mL/L    
7 MWC Metal Solution 5 mL/L    
8 Barley grains autoclaved 1 grain/10 mL