Malt Medium

Malt Medium Recipe

Suitable for all Prototheca cultures.



For 500 mL Total

  1. To approximately 500 mL of dH2O add the following component.
    • *For 1.5% agar medium add 7.5 grams of agar into the flask; do not mix.
  2. Cover and autoclave medium for no longer than 15 minutes to avoid caramelizing sugars.
  3. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Malt extract (Sigma M-0383) 33.6 g/500 mL dH2O