Liver Extract Infusion Component Recipe

Directions

For 50 mL Total

  1. To approximately 40 mL of dH2O, add 2.5 g of BACTO LIVER while stirring continuously.
  2. Bring the total volume to 50 mL.
  3. Cover the flask, and place it into a 50¡ C water bath for approximately 1 hour.
  4. Transfer the flask into an 80 ¡C water bath for 3-5 minutes.
  5. Filter extract.
# Component Amount Stock Solution Concentration Final Concentration
1 Liver extract
(Difco Bacto 0133)
2.5 g/50 mL