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Fe Component

Directions

For 200 mL total

  1. To approximately 150 mL of dH2O, add the following components in the order listed while stirring continuously.
  2. Bring volume to 200 mL with dH2O.
  3. Autoclave to sterilize.
  4. Store at refrigerator temperature.
# Component Amount Stock Solution Concentration Final Concentration
1 Fe Citrate
(Sigma F 6129)		 0.12 g/200 mL 2.5 mM
2 Citric Acid•H2O
(Fisher A 104)		 0.12 g/200 mL 2.9 mM
3 Na2EDTA•2H2O
(Sigma ED255)		 0.02 g/200 mL 1.6 mM

Note: Final concentration listed is for the stock solution.