Desmid Medium



Desmid Medium Recipe

Directions 
Modification of Pringsheim's desmidiacean medium.

For 1 L Total

1. To approximately 900 mL of dH2O add each of the components in the order specified while stirring continuously.
2. Bring total volume to 1 L with dH2O.
*For 1.5% agar medium pour 15 g of agar into the flask; do not mix.
3. Cover and autoclave medium.
4. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 Soilwater: GR+ Medium 50 mL/L    
2 MgSO4·7H2O (Sigma 230391) 10 mL/L 0.2 g/200 mL dH2O 0.04 mM
3 K2HPO4 (Sigma P 3786) 10 mL/L 0.2 g/200 mL dH2O 0.057 mM
4 KNO3 (Baker 3190) 10 mL/L 2 g/200 mL dH2O 0.99 mM