Desmid Medium



Desmid Medium Recipe

Modification of Pringsheim's desmidiacean medium.

 

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 Liter with dH2O.
    • *For 1.5% agar medium, add 15 grams of agar into the flask; do not mix.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 Soilwater: GR+ Medium 50 mL/L  
2 MgSO4·7H2O (Sigma 230391) 10 mL/L 0.2 g/200 mL dH2O 0.04 mM
3 K2HPO4 (Sigma P 3786) 10 mL/L 0.2 g/200 mL dH2O 0.057 mM
4 KNO3 (Baker 3190) 10 mL/L 2 g/200 mL dH2O 0.99 mM