For 100 mL Total
1. To approximately 85 mL of dH2O add Tricine while stirring continuously.
2. Adjust the pH to 8.0.
3. Add each of the remaining components in the order listed.
4. Bring total volume to 100 mL with dH2O.
5. Sterilize the vitamin cocktail by filtration through a 0.45 µm Millipore filter.
6. Store in the dark at freezer temperature.