Cyanidium Medium



Cyanidium Medium Recipe

Directions 
Modification of the SAG medium. Suitable for 2393 Cyanidium caldarium.

For 500 mL Total

1. To approximately 200 mL of dH2O add each of the components in the order specified while stirring continuously.
2. Adjust the pH to 2.7.
3. Continue recipe for agar or liquid.

For agar:
* a. Bring the total volume to 250 mL with dH2O.
* b. In a separate container add 7.5 g of agar into 250 mL of dH2O, do not mix.
* c. Steam both solutions for 15 minutes and put in a 50°C water bath.
* d. Add containers together aseptically and dispense before it solidifies.

For liquid:
* a. Bring the total volume to 500 mL with dH2O.
* b. Cover and autoclave medium.
* c. Store at refrigerator temperature.

# Component Amount Stock Solution
Concentration
Final Concentration
1 (NH4)2SO4 (Fisher A 702) 0.5 g/500 mL   3.78 mM
2 K2HPO4 (Sigma P 3786) 0.01 g/500 mL   0.057 mM
3 MgSO4·7H2O (Sigma 230391) 0.01 g/500 mL   0.041 mM
4 Soilwater: GR+ Medium 15 mL