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Cyanidium Medium



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Cyanidium Medium

Modification of the SAG medium. Suitable for 2393 Cyanidium caldarium.

Directions

For 500 mL Total
  1. To approximately 200 mL of dH2O add each of the components in the order specified while stirring continuously.
  2. Adjust the pH to 2.7.
  3. Continue recipe for agar or liquid.
    • For agar:
      1. Bring the total volume to 250 mL with dH2O.
      2. In a separate container add 7.5 g of agar into 250 mL of dH2O, do not mix.
      3. Steam both solutions for 15 minutes and put in a 50 °C water bath.
      4. Add containers together aseptically and dispense before it solidifies.
    • For liquid:
      1. Bring the total volume to 500 mL with dH2O.
      2. Cover and autoclave medium.
      3. Store at refrigerator temperature.

# Component Amount Stock Solution Concentration Final Concentration
1 (NH4)2SO4
(Fisher A 702)
0.5 g/500 mL 3.78 mM
2 K2HPO4
(Sigma P 3786)
0.01 g/500 mL 0.057 mM
3 MgSO47H2O
(Sigma 230391)
0.01 g/500 mL 0.041 mM
4 Soilwater: GR+ Medium 15 mL

Important Notes To Consider:

  • The quality of water, including natural seawater, is important. The term 'dH2O' generally refers to distilled, deionized, distilled/deionized water, Milli-Q water (Millipore Corp.), etc. Natural seawater (and natural freshwater) should be obtained from a non-polluted source.
  • When dissolving chemicals, wait for the first component to dissolve before adding the second. Stirring, and sometimes the addition of heat, is often required to dissolve the chemicals efficiently. Preparation of stock solutions for frequently made algal culture media make preparing media convenient but also necessary to avoid errors from weighing very tiny amounts.
  • Attention should be given to the pH of the final medium. In most cases, if pH adjustment is required, this occurs before sterilization. Please note that autoclaving removes carbon dioxide from media lacking carbonate stabilizing buffers. This would make the medium very alkaline soon after removal from the autoclave. In these cases, you should wait approximately 24 hours for gaseous equilibrium before inoculating the medium.