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Chelated Iron Solution Recipe

Directions

For 1 L Total

This solution requires the combination of two liquid components:

Component A:

  1. Pour approximately 450 mL dH2O into a beaker.
  2. Heat the beaker in the microwave oven to near boiling.
  3. Add 10.0 g of Na2EDTA to the stirring beaker.
  4. After the EDTA fully dissolves, bring the total volume to 500 mL with dH2O.

Component B:

  1. Pour approximately 450 mL 0.1 M HCl into a beaker.
  2. Add 0.81 g of FeCl3•6H2O to the beaker.
  3. Bring the total volume to 500 mL with dH2O.
  4. Slowly add Component B to the heated Component A while stirring.
# Component Amount Stock Solution Concentration Final Concentration
1 Na2EDTA•2H2O
(Sigma ED255)		 10 g/500 mL 26 µM
2 0.1M HCl 450 mL 45 mM
3 FeCl3•6H2O
(Sigma F 1513)		 0.81 g/500 mL 3 µM

Note: Final concentration listed is for the stock solution.