Algal Trace Elements Solution Recipe

Directions

For 1 L Total

  1. To approximately 950 mL of dH2O first dissolve EDTA, then add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.


# Component Amount Stock Solution Concentration Final Concentration
1 Na2EDTA•2H2O
(Sigma ED255)
4.36 g/L 110 µM
2 FeCl36H2O
(Sigma F-1513)
1 g/L 40 µM
3 CuSO45H2O
(MCIB 3M11)
0.001 g/L 0.04 µM
4 ZnSO47H2O
(Sigma Z 0251)
0.022 g/L 0.77 µM
5 CoCl26H2O
(Sigma C-3169)
0.012 g/L 0.5 µM
6 MnCl24H2O
(Baker 2540)
0.18 g/L 9.1 µM
7 Na2MoO42H2O
(J.T. Baker 3764)
0.022 g/L 0.99 µM
8 H2SeO3
(Sigma 211176)
0.001 g/L 0.077 µM
9 Na3VO4
(Sigma 450243)
0.002 g/L 0.11 µM

Note: Final concentration listed is for the stock solution.