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Algal Trace Elements Solution Recipe

Directions

For 1 L Total

  1. To approximately 950 mL of dH2O, first dissolve Na2EDTA then add each of the components in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O.
  3. Cover and autoclave medium.
  4. Store at refrigerator temperature.
#
1
ComponentNa2EDTA•2H2O
(Sigma ED255)
Amount4.36 g/L
Final Concentration110 µM
2
ComponentFeCl3•6H2O
(Sigma F 1513)
Amount1 g/L
Final Concentration40 µM
3
ComponentCuSO4•5H2O
(MCIB 3M11)
Amount0.001 g/L
Final Concentration0.04 µM
4
ComponentZnSO4•7H2O
(Sigma Z 0251)
Amount0.022 g/L
Final Concentration0.77 µM
5
ComponentCoCl2•6H2O
(Sigma C 3169)
Amount0.012 g/L
Final Concentration0.5 µM
6
ComponentMnCl2•4H2O
(Baker 2540)
Amount0.18 g/L
Final Concentration9.1 µM
7
ComponentNa2MoO4•2H2O
(J.T. Baker 3764)
Amount0.022 g/L
Final Concentration0.99 µM
8
ComponentH2SeO3
(Sigma 211176)
Amount0.001 g/L
Final Concentration0.077 µM
9
ComponentNa3VO4
(Sigma 450243)
Amount0.002 g/L
Final Concentration0.11 µM
#
1
ComponentNa2EDTA•2H2O
(Sigma ED255)
Amount4.36 g/L
Final Concentration110 µM
2
ComponentFeCl3•6H2O
(Sigma F 1513)
Amount1 g/L
Final Concentration40 µM
3
ComponentCuSO4•5H2O
(MCIB 3M11)
Amount0.001 g/L
Final Concentration0.04 µM
4
ComponentZnSO4•7H2O
(Sigma Z 0251)
Amount0.022 g/L
Final Concentration0.77 µM
5
ComponentCoCl2•6H2O
(Sigma C 3169)
Amount0.012 g/L
Final Concentration0.5 µM
6
ComponentMnCl2•4H2O
(Baker 2540)
Amount0.18 g/L
Final Concentration9.1 µM
7
ComponentNa2MoO4•2H2O
(J.T. Baker 3764)
Amount0.022 g/L
Final Concentration0.99 µM
8
ComponentH2SeO3
(Sigma 211176)
Amount0.001 g/L
Final Concentration0.077 µM
9
ComponentNa3VO4
(Sigma 450243)
Amount0.002 g/L
Final Concentration0.11 µM
# Component Amount Stock Solution Concentration Final Concentration
1 Na2EDTA•2H2O
(Sigma ED255)		 4.36 g/L 110 µM
2 FeCl3•6H2O
(Sigma F 1513)		 1 g/L 40 µM
3 CuSO4•5H2O
(MCIB 3M11)		 0.001 g/L 0.04 µM
4 ZnSO4•7H2O
(Sigma Z 0251)		 0.022 g/L 0.77 µM
5 CoCl2•6H2O
(Sigma C 3169)		 0.012 g/L 0.5 µM
6 MnCl2•4H2O
(Baker 2540)		 0.18 g/L 9.1 µM
7 Na2MoO4•2H2O
(J.T. Baker 3764)		 0.022 g/L 0.99 µM
8 H2SeO3
(Sigma 211176)		 0.001 g/L 0.077 µM
9 Na3VO4
(Sigma 450243)		 0.002 g/L 0.11 µM

Note: Final concentration listed is for the stock solution.

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