Algal Trace Elements Solution Recipe
Directions
For 1 L Total
- To approximately 950 mL of dH2O, first dissolve Na2EDTA then add each of the components in the order specified while stirring continuously.
- Bring total volume to 1 L with dH2O.
- Cover and autoclave medium.
- Store at refrigerator temperature.
| # | Component | Amount | Stock Solution Concentration | Final Concentration |
|---|---|---|---|---|
| 1 | Na2EDTA•2H2O (Sigma ED255) |
4.36 g/L | 110 µM | |
| 2 | FeCl3•6H2O (Sigma F 1513) |
1 g/L | 40 µM | |
| 3 | CuSO4•5H2O (MCIB 3M11) |
0.001 g/L | 0.04 µM | |
| 4 | ZnSO4•7H2O (Sigma Z 0251) |
0.022 g/L | 0.77 µM | |
| 5 | CoCl2•6H2O (Sigma C 3169) |
0.012 g/L | 0.5 µM | |
| 6 | MnCl2•4H2O (Baker 2540) |
0.18 g/L | 9.1 µM | |
| 7 | Na2MoO4•2H2O (J.T. Baker 3764) |
0.022 g/L | 0.99 µM | |
| 8 | H2SeO3 (Sigma 211176) |
0.001 g/L | 0.077 µM | |
| 9 | Na3VO4 (Sigma 450243) |
0.002 g/L | 0.11 µM |
Note: Final concentration listed is for the stock solution.
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