Algal Trace Elements Solution Recipe

To approximately 950 mL of dH₂O, first dissolve Na₂EDTA then add each of the components in the order specified while stirring continuously.
Bring total volume to 1 L with dH₂O.
Cover and autoclave medium.
Store at refrigerator temperature.

#	Component	Amount	Final Concentration
1	Na₂EDTA•2H₂O (Sigma ED255)	4.36 g/L	110 µM
2	FeCl₃•6H₂O (Sigma F 1513)	1 g/L	40 µM
3	CuSO₄•5H₂O (MCIB 3M11)	0.001 g/L	0.04 µM
4	ZnSO₄•7H₂O (Sigma Z 0251)	0.022 g/L	0.77 µM
5	CoCl₂•6H₂O (Sigma C 3169)	0.012 g/L	0.5 µM
6	MnCl₂•4H₂O (Baker 2540)	0.18 g/L	9.1 µM
7	Na₂MoO₄•2H₂O (J.T. Baker 3764)	0.022 g/L	0.99 µM
8	H₂SeO₃ (Sigma 211176)	0.001 g/L	0.077 µM
9	Na₃VO₄ (Sigma 450243)	0.002 g/L	0.11 µM