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A+ Trace Components Recipe

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O add the components and stock solutions in the order specified while stirring continuously.
  2. Bring total volume to 1 Liter with dH2O at refrigerator temperature, away from bright light.
# Component Amount Stock Solution Concentration Final Concentration
1 H3BO3
(Baker 0084)		 3.43 g/L 55.5 mM
2 ZnCl2
(Sigma Z 0152)		 0.0315 g/L 0.23 mM
3 MoO3
(85%) 		0.003 g/L 0.021 mM
4 Vitamin B12
(cyanocobalamin)
(Sigma V 6629)		 0.0004 g/L 0.3 µM
5 FeCl3•6H2O
(Sigma F 1513)		 1 mL/L 3.89 g/100 mL 0.14 mM
6 MnCl2•4H2O
(Baker 2540)		 1 mL/L 4.3 g/100 mL 0.22 mM
7 CuSO4•5H2O
(MCIB 3M11)		 1 mL/L 0.003 g/100 mL 0.00012 mM
8 CoCl2•6H2O
(Sigma C 3169)		 1 mL/L 0.012 g/100 mL 0.0005 mM

Note: Final concentration listed is for the stock solution.