A+ Trace Components Recipe

Directions

For 1 L Total

  1. To approximately 900 mL of dH2O add the components and stock solutions in the order specified while stirring continuously.
  2. Bring total volume to 1 L with dH2O at refrigerator temperature, away from bright light.
# Component Amount Stock Solution Concentration Final Concentration
1 H3BO3
(Baker 0084)
3.43 g/L 55.5 mM
2 ZnCl2
(Sigma Z-0152)
0.0315 g/L 0.23 mM
3 MoO3
(85%)
0.003 g/L 0.021 mM
4 Vitamin B12
(cyanocobalamin, (Sigma V-6629))
0.0004 g/L 0.3 µM
5 FeCl36H2O
(Sigma F-1513)
1 mL/L 3.89 g/100 mL 0.14 mM
6 MnCl24H2O
(Baker 2540)
1 mL/L 4.3 g/100 mL 0.22 mM
7 CuSO45H2O
(MCIB 3M11)
1 mL/L 0.003 g/100 mL 0.00012 mM
8 CoCl26H2O
(Sigma C-3169)
1 mL/L 0.012 g/100 mL 0.0005 mM

Note: Final concentration listed is for the stock solution.