2X CHEV Diatom Medium
The medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps.
For routine maintenance and transfer, autoclaved media for 15 min, since transfer material typically contains organics and/or bacteria needed for algal growth. In pasteurization, tubes are brought to just over 98 °C, and held at that temperature for 2 hrs. They are then allowed to cool to room temperature for 20-24 hrs. This process is repeated for three successive days. It is imperative that media (pasteurized or autoclaved) be allowed to gas-equilibrate for 2 days prior to use.
Add the components in the following order. Once soil and liquid are mixed, the media must be processed (pasteurized or autoclaved) within 6 hrs.
||Stock Solution Concentration
||MgCO3 (MCIB CB486)
||0.5 mg/12 mL