2X CHEV Diatom Medium

Upcoming Order Deadlines:

Orders for algal culture media placed after 5:00 PM CST on Wednesday, December 12th, 2018 through Wednesday, January 2nd, 2019, will not ship until Monday, January 7th, 2019. UTEX will be closed for the 2018 Winter Break from December 24, 2018 - January 1, 2019. Please click here to view more information .

2X CHEV Diatom Medium Recipe

The medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps.

For routine maintenance and transfer, autoclaved media for 15 min, since transfer material typically contains organics and/or bacteria needed for algal growth. In pasteurization, tubes are brought to just over 98°C, and held at that temperature for 2 hrs. They are then allowed to cool to room temperature for 20-24 hrs. This process is repeated for three successive days. It is imperative that media (pasteurized or autoclaved) be allowed to gas-equilibrate for 2 days prior to use.


Add the components in the following order. Once soil and liquid are mixed, the media must be processed (pasteurized or autoclaved) within 6 hours.

# Component Amount Stock Solution Concentration Final Concentration
1 CR1 Soil 1 cc    
2 MgCO3 (MCIB CB486) 0.5 mg/12 mL   0.5 mM
3 dH2O 10 mL    
4 Pasteurized Seawater 2 mL