2X CHEV Diatom Medium



2X CHEV Diatom Medium Recipe

The medium is prepared on a tube by tube basis, using 15 mL glass tubes, stoppered with rubber-lined screw caps.

For routine maintenance and transfer, autoclaved media for 15 min, since transfer material typically contains organics and/or bacteria needed for algal growth. In pasteurization, tubes are brought to just over 98°C, and held at that temperature for 2 hrs. They are then allowed to cool to room temperature for 20-24 hrs. This process is repeated for three successive days. It is imperative that media (pasteurized or autoclaved) be allowed to gas-equilibrate for 2 days prior to use.

Directions

Add the components in the following order. Once soil and liquid are mixed, the media must be processed (pasteurized or autoclaved) within 6 hours.


# Component Amount Stock Solution Concentration Final Concentration
1 CR1 Soil 1 cc    
2 MgCO3 (MCIB CB486) 0.5 mg/12 mL   0.5 mM
3 dH2O 10 mL    
4 Pasteurized Seawater 2 mL