Add a source of silica to culture media that is lacking the component. Often used when culturing diatoms or any strains that require silica. One bottle can provide a 200 µM silica boost to approximately 125 Liters of media*.
Sodium Metasilicate Solution At A Glance
|Total Volume||125 mL|
|*Amount to add||Add 1 mL per Liter for a 200 µM final concentration|
Metasilicate is required for many diatom strains and is important for the formation of frustules. Diatoms need about the same ratio of nitrogen and silicon for growth, with evidence showing a lower growth rate of diatoms in media containing ratios with less silicon versus nitrogen.
Add 1 mL/L of this solution to your media to provide a boost of silica (Final Concentration: 200 µM). The Sodium Metasilicate Add-In Solution is sterile and should be handled aseptically to prevent contamination.