The basic garden-type soilwater; includes a pinch of CaCO3, which is added to the soil and water prior to steaming; suitable for most phototrophic freshwater algae. Optional ingredients: add vitamin B12 to cultures of Volvox; a pinch of NH4MgPO4•6H2O added to soilwater cultures of Botryococcus, Synechococcus and some Euglenoids enhances growth and to LB 826 Gonium pectorale increases the numbers of 16-celled colonies formed.
For 200 mL Total
|#||Component||Amount||Stock Solution Concentration||Final Concentration|
|1||Green House Soil||1 tsp|
|2||CaCO3 (optional) (Fisher C 64)||1 mg||0.05 mM|