2X Soil+Seawater Medium
For marine algae that require salinities at 60 ppt. Add 1 L of Supplemented Pasteurized Seawater divided into 200 mL volumes and combined with soil in preparation for pasteurizing with steam.
For 200 mL Total
- Combine all components.
- Cover the medium container and steam for 2 consecutive days, 3 hours on each day. Pasteurization is a gradual rising of temperature to approximately 95 °C in 15 minutes. Then increased just over 98 °C for the 3 hour duration. Cooling occurs gradually at room temperature.
- Refrigerate 24 hours or more and bring to room temperature before use.
||Stock Solution Concentration
||Green House Soil
||5 mL/200 mL of Supplemented Seawater
(Fisher C 64)
|1 mg/200 mL of Supplemented Seawater