WC Trace Elements Solution Recipe


WC Trace Elements Solution Recipe

Directions

Algal Trace Elements Solution for WC Medium.

1. To approximately 950 mL of dd-H2O, first dissolve EDTA and then add the remaining components in order.
2. Transfer the contents of the beaker to a 1-Liter graduated cylinder and bring the total volume to 1.0 Liter with dd-H2O.
3. Store in the refrigerator.

# Component Amount Stock Solution
Concentration
Final Concentration
1 Na2EDTA2H2O (Sigma ED255) 4.36 g/L 11.7 µM
2 FeCl36H2O (Sigma F-1513) 3.15 g/L 11.7 µM
3 CuSO45H2O (MCIB 3M11) 1 mL/L 2.5 g/L 10 nM
4 ZnSO47H2O (Sigma Z 0251) 1 mL/L 22 g/L 76.5 nM
5 CoCl26H2O (Sigma C-3169) 1 mL/L 10 g/L 42 nM
6 MnCl24H2O (Baker 2540) 1 mL/L 180 g/L 910 nM
7 Na2MoO42H2O (J.T. Baker 3764) 1 mL/L 6.3 g/L 26 nM
8 Na3VO4 (Sigma 450243) 1 mL/L 18 g/L 98 nM

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.