Vitamin B12 Solution Component Recipe


Vitamin B12 Solution Component Recipe

Directions

For 200 mL Total

  1. Prepare 200 mL of HEPES buffer (50 mM).
  2. Adjust the pH to 7.8.
  3. Add Vitamin B12 (0.1 mM) wait until fully dissolved.
  4. Sterilize by 0.45 µm Millipore filter. Store in dark at freezer temperature.
    • The amount of vitamins added can vary from medium to medium so the final concentration is not listed.

Note final concentration listed is for the stock solution.


# Component Amount Stock Solution Concentration Final Concentration
1 HEPES buffer pH 7.8 (Sigma H-3375) 2.4 g/200 mL dH2O
2 Vitamin B12 (cyanocobalamin, (Sigma V-6629)) 0.027 g/200 mL dH2O

UTEX only sells complete media at 1X concentration. Stock solutions and individual components are currently not available for purchase.